PMID- 9873069
OWN - NLM
STAT- MEDLINE
DCOM- 19990114
LR  - 20111117
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 4
DP  - 1998 Dec 31
TI  - Familial subepithelial corneal amyloidosis (gelatinous drop-like corneal 
      dystrophy): exclusion of linkage to lactoferrin gene.
PG  - 31
AB  - PURPOSE: Because corneal tissue with familial subepithelial corneal amyloidosis 
      (FSCA; gelatinous drop-like dystrophy of the cornea) contains lactoferrin the 
      possibility that the FSCA gene was the human lactoferrin (hLF) gene was 
      investigated. Due to contradictory published information we also mapped the hLF 
      gene. METHODS: We mapped the hLF gene using a genomic clone of the entire hLF 
      gene as a probe by fluorescence in situ hybridization (FISH). Utilizing PCR 
      primers that are specific to the hLF gene, we also mapped the hLF via radiation 
      somatic cell hybrid analysis. Linkage of the FSCA gene to the hLF gene was 
      evaluated by genetic linkage analysis using polymorphic markers within and in the 
      vicinity of the hLF gene. RESULTS: The hLF gene mapped to the short arm of 
      chromosome 3 at 3p21. Linkage analysis using polymorphic markers for hLF and 
      haplotype analysis of the 3p21 loci indicates that the FSCA gene is not linked to 
      the 3p21 locus. CONCLUSIONS: The gene for FSCA is not the hLF gene in these 
      families.
FAU - Klintworth, G K
AU  - Klintworth GK
AD  - Department of Pathology, Duke University, Durham, NC 27710, USA. 
      KLINT001@mc.duke.edu
FAU - Sommer, J R
AU  - Sommer JR
FAU - Obrian, G
AU  - Obrian G
FAU - Han, L
AU  - Han L
FAU - Ahmed, M N
AU  - Ahmed MN
FAU - Qumsiyeh, M B
AU  - Qumsiyeh MB
FAU - Lin, P Y
AU  - Lin PY
FAU - Basti, S
AU  - Basti S
FAU - Reddy, M K
AU  - Reddy MK
FAU - Kanai, A
AU  - Kanai A
FAU - Hotta, Y
AU  - Hotta Y
FAU - Sugar, J
AU  - Sugar J
FAU - Kumaramanickavel, G
AU  - Kumaramanickavel G
FAU - Munier, F
AU  - Munier F
FAU - Schorderet, D F
AU  - Schorderet DF
FAU - El Matri, L
AU  - El Matri L
FAU - Iwata, F
AU  - Iwata F
FAU - Kaiser-Kupfer, M
AU  - Kaiser-Kupfer M
FAU - Nagata, M
AU  - Nagata M
FAU - Nakayasu, K
AU  - Nakayasu K
FAU - Hejtmancik, J F
AU  - Hejtmancik JF
FAU - Teng, C T
AU  - Teng CT
LA  - eng
GR  - EY 00146/EY/NEI NIH HHS/United States
GR  - P30 EY5722/EY/NEI NIH HHS/United States
GR  - R01EY 08249/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 19981231
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - EC 3.4.21.- (Lactoferrin)
SB  - IM
MH  - Amyloidosis/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 3
MH  - Corneal Dystrophies, Hereditary/*genetics
MH  - Female
MH  - Genetic Linkage
MH  - Haplotypes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lactoferrin/*genetics
MH  - Male
MH  - Pedigree
MH  - Polymorphism, Genetic
MH  - Polymorphism, Single-Stranded Conformational
EDAT- 1999/01/05 00:00
MHDA- 1999/01/05 00:01
CRDT- 1999/01/05 00:00
PHST- 1999/01/05 00:00 [pubmed]
PHST- 1999/01/05 00:01 [medline]
PHST- 1999/01/05 00:00 [entrez]
PST - epublish
SO  - Mol Vis. 1998 Dec 31;4:31.