PMID- 9874497
OWN - NLM
STAT- MEDLINE
DCOM- 19990122
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 48
IP  - 6
DP  - 1998 Dec
TI  - Effect of allergen-specific immunotherapy on interleukin-4, interleukin-5 and 
      interferon-gamma mRNA expression in the nasal mucosa of rats with allergic 
      rhinitis.
PG  - 629-34
AB  - To elucidate the mechanism of immunotherapy, we tested the effect of ovalbumin 
      and ovalbumin-pullulan conjugate immunotherapy on the expression of interleukin 
      (IL)-4, IL-5 and interferon-gamma (IFN-gamma) mRNA in the nasal mucosa of 
      sensitized rats. Forty-five rats were injected with ovalbumin intraperitoneally 
      on three consecutive days and later were exposed to ovalbumin aerosol. The rats 
      were injected intradermally, on six consecutive days, with saline, ovalbumin or 
      ovalbumin-pullulan conjugate. Later, nasal mucosa was obtained and reverse 
      transcription-polymerase chain reaction (RT-PCR) was performed. Nasal responses 
      and specific immunoglobulin E (IgE) were measured. Although the immunotherapy 
      significantly decreased nasal airway resistance, dye leakage and histamine 
      content in nasal irrigation after allergen challenge, no significant difference 
      was found in IL-4 and IL-5 mRNA expression or in specific IgE level among the 
      three groups. We conclude that in this allergic model, the improvement of nasal 
      responses after immunotherapy was the result of a mechanism other than decrease 
      of T-helper 2 (Th2) cytokines.
FAU - El-Naggar, M M
AU  - El-Naggar MM
AD  - Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura 
      University, Egypt.
FAU - Ukai, K
AU  - Ukai K
FAU - Takeuchi, K
AU  - Takeuchi K
FAU - Sakakura, Y
AU  - Sakakura Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Allergens)
RN  - 0 (Interleukin-5)
RN  - 0 (RNA, Messenger)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Airway Resistance/immunology
MH  - Allergens/*immunology
MH  - Animals
MH  - Blotting, Southern
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression
MH  - Histamine/metabolism
MH  - Immunoglobulin E/blood
MH  - Immunotherapy
MH  - Interferon-gamma/*genetics
MH  - Interleukin-4/*genetics
MH  - Interleukin-5/*genetics
MH  - Male
MH  - Nasal Mucosa/metabolism
MH  - Ovalbumin/*immunology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rhinitis/chemically induced/metabolism
EDAT- 1999/01/05 00:00
MHDA- 1999/01/05 00:01
CRDT- 1999/01/05 00:00
PHST- 1999/01/05 00:00 [pubmed]
PHST- 1999/01/05 00:01 [medline]
PHST- 1999/01/05 00:00 [entrez]
AID - 10.1046/j.1365-3083.1998.00449.x [doi]
PST - ppublish
SO  - Scand J Immunol. 1998 Dec;48(6):629-34. doi: 10.1046/j.1365-3083.1998.00449.x.