PMID- 9875636
OWN - NLM
STAT- MEDLINE
DCOM- 19990323
LR  - 20190817
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 29
IP  - 6
DP  - 1998 Dec
TI  - Expression and regulation of intercellular adhesion molecule-1 (ICAM-1) in 
      organotypic cultures of rat liver tissue.
PG  - 901-9
AB  - BACKGROUND/AIMS: The objective of the present study was to analyze the expression 
      and regulation of intercellular adhesion molecule-1 (ICAM-1) in organotypic 
      cultures of rat liver slices, which preserve the normal microenvironment of liver 
      cells. METHODS: Rat liver slices were maintained in culture for 15 min to 24 h 
      and examined for ICAM-1 expression by immunohistochemistry and Western blotting 
      in basal conditions and after stimulation with 1000 IU/ml interferon-gamma 
      (IFNgamma), 1000 IU/ml tumor necrosis factor-alpha (TNF alpha) and 50 microg/ml 
      endotoxin. Immunohistochemical results were evaluated using a semiquantitative 
      scoring system. RESULTS: In uncultured slices, ICAM-1 was not detected on 
      hepatocytes. In unstimulated liver slices maintained in organotypic culture, 
      ICAM-1 was induced at the surface of scattered hepatocytes (score at 15 min, 
      0.33+/-0.47 and at 24 h, 1.17+/-0.69). After 4 h of stimulation, a significant 
      increase in ICAM-1 expression by hepatocytes and adjacent sinusoidal cells, but 
      not by intra-hepatic biliary epithelial cells, was observed for IFNgamma (score: 
      2.35+/-0.47) and endotoxin (score: 2.67+/-0.47), but not with TNF alpha (score: 
      0.66+/-0.47). After 24 h of stimulation, a further increase in the extent of 
      ICAM-1 expression by hepatocytes was observed for IFNgamma (score: 3.67+/-0.47) 
      and endotoxin (score: 4.0+/-0.0), and a significant overexpression of ICAM-1 by 
      hepatocytes was detectable after treatment with TNF alpha (score: 3.67+/-0.47). 
      CONCLUSIONS: In rat liver organotypic cultures, TNF alpha, IFNgamma and endotoxin 
      induce the expression of ICAM-1 in hepatocytes and adjacent sinusoidal 
      endothelial cells, but not in portal tracts.
FAU - Oudar, O
AU  - Oudar O
AD  - Laboratoire de Biologie Cellulaire, INSERM U.327, Faculte de Medecine 
      Xavier-Bichat, Paris, France.
FAU - Moreau, A
AU  - Moreau A
FAU - Feldmann, G
AU  - Feldmann G
FAU - Scoazec, J Y
AU  - Scoazec JY
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (Endotoxins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Endotoxins/pharmacology
MH  - Immunohistochemistry
MH  - Intercellular Adhesion Molecule-1/biosynthesis/*physiology
MH  - Interferon-gamma/pharmacology
MH  - Liver/cytology/*drug effects/metabolism
MH  - Organ Culture Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1999/01/06 00:00
MHDA- 1999/01/06 00:01
CRDT- 1999/01/06 00:00
PHST- 1999/01/06 00:00 [pubmed]
PHST- 1999/01/06 00:01 [medline]
PHST- 1999/01/06 00:00 [entrez]
AID - S0168-8278(98)80117-7 [pii]
AID - 10.1016/s0168-8278(98)80117-7 [doi]
PST - ppublish
SO  - J Hepatol. 1998 Dec;29(6):901-9. doi: 10.1016/s0168-8278(98)80117-7.