PMID- 9876834
OWN - NLM
STAT- MEDLINE
DCOM- 19990323
LR  - 20131121
IS  - 0005-2086 (Print)
IS  - 0005-2086 (Linking)
VI  - 42
IP  - 4
DP  - 1998 Oct-Dec
TI  - Efficacy of a temperature-sensitive Mycoplasma synoviae live vaccine.
PG  - 671-6
AB  - A temperature-sensitive clone of Mycoplasma synoviae (MS), MS-H, derived from the 
      chemical mutagenesis of the Australian field isolate 86079/7NS, was investigated 
      for efficacy as a live vaccine. Titers of MS-H vaccine ranged between 1.16 x 
      10(8) and 8.4 x 10(8) color changing units/ml when incubated at 33 C and were 
      consistently > or = 10(2) lower when incubated at 39.5 C. Laboratory-produced 
      MS-H vaccine protected 8 out of 10 specific-pathogen-free Webster white leghorn 
      chickens against a combined experimental challenge of aerosols of the virulent 
      wild MS strain 88064/FP and T-strain infectious bronchitis vaccine administered 
      intratracheally. Laboratory and commercially produced vaccines were compared for 
      efficacy against thoracic air sac challenge, with both achieving similar levels 
      of air sac protection. Air sac lesion incidences of five air sacs with lesions 
      out of 32 and four out of 32 were seen in groups vaccinated with laboratory and 
      commercial vaccines, respectively, compared with 13 out of 20 in nonvaccinated 
      specific-pathogen-free hybrid white leghorn chickens. An attempt to determine the 
      dose response of the commercial vaccine was conducted with 0.5, 2, and 4 times 
      the standard dose. Air sac lesion incidences of 16 air sacs with lesions out of 
      32 in the 0.5 times, 11 out of 32 in the 2 times, and 1 out of 32 in the 4 times 
      dose groups were observed.
FAU - Markham, J F
AU  - Markham JF
AD  - School of Veterinary Science, University of Melbourne, Parkville, Victoria, 
      Australia.
FAU - Morrow, C J
AU  - Morrow CJ
FAU - Whithear, K G
AU  - Whithear KG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Avian Dis
JT  - Avian diseases
JID - 0370617
RN  - 0 (Bacterial Vaccines)
RN  - 12H3O2UGSF (Methylnitronitrosoguanidine)
SB  - IM
MH  - Air Sacs/microbiology/pathology
MH  - Animals
MH  - *Bacterial Vaccines
MH  - Chickens
MH  - Coronavirus Infections/complications/immunology/veterinary
MH  - Infectious bronchitis virus
MH  - Methylnitronitrosoguanidine
MH  - Mutagenesis
MH  - Mycoplasma/genetics/growth & development/*immunology
MH  - Mycoplasma Infections/immunology/prevention & control/*veterinary
MH  - Poultry Diseases/*immunology/prevention & control
MH  - Specific Pathogen-Free Organisms
MH  - Temperature
EDAT- 1999/01/07 00:00
MHDA- 1999/01/07 00:01
CRDT- 1999/01/07 00:00
PHST- 1999/01/07 00:00 [pubmed]
PHST- 1999/01/07 00:01 [medline]
PHST- 1999/01/07 00:00 [entrez]
PST - ppublish
SO  - Avian Dis. 1998 Oct-Dec;42(4):671-6.