PMID- 9878387
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20061115
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 285
IP  - 1
DP  - 1999 Jan 8
TI  - Delineation of a membrane-proximal beta-rich domain in the GABAA receptor by 
      progressive deletions.
PG  - 55-61
AB  - The type A gamma-aminobutyric acid (GABAA) receptor plays a major inhibitory role 
      in the central nervous system. Structural elucidation of the GABAA receptor has 
      been impeded by the large size of the receptor. We present here the delineation 
      of a minimal structural domain as the first step of dissecting the receptor 
      structure. This was achieved through prediction-assisted progressive deletions: 
      the prediction of a candidate structural domain rich in beta-strands with no 
      close similarity to known structures was tested by deleting putative secondary 
      structure elements from the ends of the proposed domain, as well as mutations 
      within the terminal secondary structures. Such progressive deletions revealed the 
      limits of an integral domain, spanning Cys180 to Met293 (numbering of human 
      alpha1 subunit). Below these limits the intact domain structure, as indicated by 
      its circular dichroism, collapses. Based on its putative position, this domain is 
      provisionally designated the membrane-proximal beta-rich domain of GABAA 
      receptor. The inclusion of sequences from the first two out of four previously 
      suggested transmembrane segments and one of the two conserved Cys residues in 
      this domain defines important constraints to the receptor structure.
CI  - Copyright 1999 Academic Press.
FAU - Xue, H
AU  - Xue H
AD  - Department of Biochemistry, Hong Kong University of Science and Technology, Clear 
      Water Bay, Hong Kong. hxue@usthk.ust.hk
FAU - Hang, J
AU  - Hang J
FAU - Chu, R
AU  - Chu R
FAU - Xiao, Y
AU  - Xiao Y
FAU - Li, H
AU  - Li H
FAU - Lee, P
AU  - Lee P
FAU - Zheng, H
AU  - Zheng H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (Receptors, GABA-A)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Cell Membrane/chemistry/metabolism
MH  - Circular Dichroism
MH  - Humans
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Mutagenesis
MH  - Protein Structure, Secondary
MH  - Receptors, GABA-A/*chemistry/genetics/metabolism
MH  - Sequence Deletion
MH  - Ultraviolet Rays
EDAT- 1999/01/08 00:00
MHDA- 1999/01/08 00:01
CRDT- 1999/01/08 00:00
PHST- 1999/01/08 00:00 [pubmed]
PHST- 1999/01/08 00:01 [medline]
PHST- 1999/01/08 00:00 [entrez]
AID - S0022-2836(98)92317-8 [pii]
AID - 10.1006/jmbi.1998.2317 [doi]
PST - ppublish
SO  - J Mol Biol. 1999 Jan 8;285(1):55-61. doi: 10.1006/jmbi.1998.2317.