PMID- 9878688
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 816
IP  - 1
DP  - 1999 Jan 16
TI  - In the ventromedial nucleus of the rat hypothalamus, GABA-immunolabeled neurons 
      are abundant and are innervated by both enkephalin- and GABA-immunolabeled axon 
      terminals.
PG  - 58-67
AB  - Immunohistochemical-labeling for the neurochemicals gamma-aminobutyric acid 
      (GABA) and enkephalin are abundant in the ventromedial nucleus of the 
      hypothalamus (VMN). In VMN, both GABA and enkephalin may function to regulate 
      feeding behavior, as well as other hormone-controlled behaviors. Importantly, in 
      several brain areas, enkephalin is often thought to modulate GABAergic 
      neurotransmission. Therefore, we used dual-labeling immunohistochemistry with 
      electron microscopic analysis to study the circuitry of neurons containing GABA- 
      and/or enkephalin-labeling within the VMN. Somato-dendritic profiles containing 
      GABA-labeling were three fold more abundant than GABA-labeled axon terminals (117 
      soma or dendrites vs. 34 axons). In addition, axon terminals containing 
      GABA-labeling sometimes synapsed onto GABA-labeled somata or dendrites (25% or 
      9/34). In contrast, under these conditions labeling for enkephalin was primarily 
      restricted to axon terminals, which were very abundant throughout VMN. 
      Enkephalin-containing terminals accounted for a large fraction (25% 23/92) of the 
      axons in contact with GABA-labeled dendrites, although they also contacted 
      unlabeled dendrites. These observations suggest that a population of VMN neurons 
      are GABAergic. These may be either local circuit 'interneurons' or projection 
      neurons. In addition, GABA-labeled VMN neurons may be regulated by either 
      enkephalin or GABA. These morphologic observations provide the basis for 
      disinhibitory mechanisms to function within the VMN.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Commons, K G
AU  - Commons KG
AD  - Laboratory of Neurobiology and Behavior, The Rockefeller University Box 275, 1230 
      York Avenue, New York, NY 10021, USA.
FAU - Kow, L M
AU  - Kow LM
FAU - Milner, T A
AU  - Milner TA
FAU - Pfaff, D W
AU  - Pfaff DW
LA  - eng
GR  - DA08259/DA/NIDA NIH HHS/United States
GR  - DK 07313-18/DK/NIDDK NIH HHS/United States
GR  - NS60824/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Enkephalins)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Animals
MH  - Axons/metabolism/ultrastructure
MH  - Dendrites/metabolism/ultrastructure
MH  - Enkephalins/*metabolism
MH  - Immunohistochemistry
MH  - Interneurons/metabolism
MH  - Male
MH  - Microscopy, Electron
MH  - Neurons/*metabolism/physiology/ultrastructure
MH  - Presynaptic Terminals/*metabolism/ultrastructure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Synapses/metabolism/ultrastructure
MH  - Ventromedial Hypothalamic Nucleus/*metabolism
MH  - gamma-Aminobutyric Acid/*metabolism
EDAT- 1999/01/08 00:00
MHDA- 1999/01/08 00:01
CRDT- 1999/01/08 00:00
PHST- 1999/01/08 00:00 [pubmed]
PHST- 1999/01/08 00:01 [medline]
PHST- 1999/01/08 00:00 [entrez]
AID - S0006-8993(98)01084-1 [pii]
AID - 10.1016/s0006-8993(98)01084-1 [doi]
PST - ppublish
SO  - Brain Res. 1999 Jan 16;816(1):58-67. doi: 10.1016/s0006-8993(98)01084-1.