PMID- 9879541
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20190516
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 60
IP  - 12
DP  - 1998 Dec
TI  - Evaluation of equine infectious anemia virus core proteins produced in a 
      baculovirus expression system in agar gel immunodiffusion test and enzyme-linked 
      immunosorbent assay.
PG  - 1361-2
AB  - Equine infectious anemia virus (EIAV) core proteins (Gag and p26) obtained from a 
      baculovirus expression system were used in agar gel immunodiffusion (AGID) and 
      enzyme-linked immunosorbent assay (ELISA) antigens to test seventy-six horse 
      sera. Those sera showed false-positive reaction in AGID test using Nisseiken 
      antigen. However, none of them showed false-positive reaction with both of the 
      expressed antigens. The 76 horse sera were also tested by ELISA. The sera gave a 
      high background in ELISA using Nisseiken antigen. Gag and p26 reacted strongly 
      against positive sera from horses immunized with Nisseiken antigen.
FAU - Kong, X G
AU  - Kong XG
AD  - National Institute of Animal Health, Ibaraki, Japan.
FAU - Pang, H
AU  - Pang H
FAU - Sugiura, T
AU  - Sugiura T
FAU - Matsumoto, Y
AU  - Matsumoto Y
FAU - Onodera, T
AU  - Onodera T
FAU - Akashi, H
AU  - Akashi H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Antibodies, Viral)
RN  - 0 (Gene Products, gag)
RN  - 0 (Viral Core Proteins)
RN  - 0 (equine infectious anemia virus, p26)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/analysis
MH  - Baculoviridae
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Equine Infectious Anemia/*diagnosis
MH  - Gene Products, gag/*immunology
MH  - Horses
MH  - Immunodiffusion/veterinary
MH  - Infectious Anemia Virus, Equine/*immunology
MH  - Spodoptera
MH  - Viral Core Proteins/*immunology
EDAT- 1999/01/08 00:00
MHDA- 1999/01/08 00:01
CRDT- 1999/01/08 00:00
PHST- 1999/01/08 00:00 [pubmed]
PHST- 1999/01/08 00:01 [medline]
PHST- 1999/01/08 00:00 [entrez]
AID - 10.1292/jvms.60.1361 [doi]
PST - ppublish
SO  - J Vet Med Sci. 1998 Dec;60(12):1361-2. doi: 10.1292/jvms.60.1361.