PMID- 9879835
OWN - NLM
STAT- MEDLINE
DCOM- 19990312
LR  - 20190905
IS  - 0340-3696 (Print)
IS  - 0340-3696 (Linking)
VI  - 290
IP  - 12
DP  - 1998 Dec
TI  - Expression of vascular endothelial growth factor (VEGF) in various compartments 
      of the human hair follicle.
PG  - 661-8
AB  - Hair follicle vascularization appears to be closely related to the processes 
      involved in hair cycle regulation, in which growth factors, cytokines and other 
      bioactive molecules are involved. In particular, vascular endothelial growth 
      factor (VEGF), essential for angiogenesis and vascular permeability, may be 
      responsible for maintaining proper vasculature around the hair follicle during 
      the anagen growth phase. The aim of our study was to compare the in vitro 
      angiogenic capacity, i.e. the steady-state expression of the VEGF gene, of 
      different cultured cell types derived from normal human hair follicles, 
      corresponding to different follicular compartments. Human dermal papilla cells 
      (DPC), fibrous sheath fibroblasts, dermal fibroblasts, and follicular and 
      interfollicular keratinocytes were cultured and studied in vitro for VEGF 
      expression at the mRNA level using RT-PCR, and for VEGF protein synthesis by 
      radioimmunoprecipitation and immunocytochemistry. In vivo examination for VEGF 
      expression in human terminal hair follicles was performed using 
      immunohistochemical methods. In the present report the expression of four 
      different VEGF molecular isoforms, differing in their angiogenic capacity, are 
      described in different cultured follicular cell types for the first time. 
      Cultured follicular cells strongly expressed mRNA of four VEGF molecular species 
      identified as the 121-, 145-, 165- and 189-amino acid splice variants, the most 
      prominent being the 121-amino acid molecule. DPC, and also other mesenchymal 
      cells such as fibrous sheath fibroblasts and dermal fibroblasts, in vivo and in 
      vitro strongly expressed VEGF mRNA and synthesized a 46-kDa VEGF protein, whereas 
      follicular and interfollicular keratinocytes in vitro expressed lower levels of 
      VEGF mRNA and proteins than mesenchymal cells. As the highest expression of VEGF 
      was found in DPC, we suggest that DPC are mainly responsible for angiogenic 
      processes possibly related to the human hair cycle.
FAU - Kozlowska, U
AU  - Kozlowska U
AD  - Department of Dermatology, University Medical Center Benjamin Franklin, The Free 
      University of Berlin, Germany.
FAU - Blume-Peytavi, U
AU  - Blume-Peytavi U
FAU - Kodelja, V
AU  - Kodelja V
FAU - Sommer, C
AU  - Sommer C
FAU - Goerdt, S
AU  - Goerdt S
FAU - Majewski, S
AU  - Majewski S
FAU - Jablonska, S
AU  - Jablonska S
FAU - Orfanos, C E
AU  - Orfanos CE
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Arch Dermatol Res
JT  - Archives of dermatological research
JID - 8000462
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
SB  - IM
MH  - Cells, Cultured
MH  - Endothelial Growth Factors/*analysis/genetics/physiology
MH  - Fibroblasts/metabolism
MH  - Hair Follicle/blood supply/*chemistry
MH  - Humans
MH  - Immunohistochemistry
MH  - Lymphokines/*analysis/genetics/physiology
MH  - *Neovascularization, Physiologic
MH  - RNA Splicing
MH  - RNA, Messenger/analysis
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 1999/01/08 00:00
MHDA- 1999/01/08 00:01
CRDT- 1999/01/08 00:00
PHST- 1999/01/08 00:00 [pubmed]
PHST- 1999/01/08 00:01 [medline]
PHST- 1999/01/08 00:00 [entrez]
AID - 10.1007/s004030050370 [doi]
PST - ppublish
SO  - Arch Dermatol Res. 1998 Dec;290(12):661-8. doi: 10.1007/s004030050370.