PMID- 9880013
OWN - NLM
STAT- MEDLINE
DCOM- 19990129
LR  - 20061115
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 79 ( Pt 12)
DP  - 1998 Dec
TI  - A pneumo-virulent United States isolate of porcine reproductive and respiratory 
      syndrome virus induces apoptosis in bystander cells both in vitro and in vivo.
PG  - 2989-95
AB  - Evidence of apoptosis was detected for the United States porcine reproductive and 
      respiratory syndrome virus (PRRSV) in ATCC CRL11171 cells inoculated with strain 
      ATCC VR2385 and in the tissues of pigs infected with the same strain. Apoptosis 
      was detected by agarose gel electrophoresis, transmission electron microscopy and 
      terminal deoxytransferase dUTP nick end labelling (TUNEL) techniques. By electron 
      microscopy and double-labelling techniques, apoptosis was detected primarily in 
      uninfected bystander cells in the continuous cell line rather than the 
      PRRSV-infected cells. In the lungs, the apoptotic cells were predominantly 
      alveolar and pulmonary intravascular macrophages, and mononuclear cells in the 
      alveolar septa. In the lymph nodes, the apoptotic cells were predominantly 
      tingible body macrophages and mononuclear cells. The induction of apoptosis in a 
      large number of mononuclear cells in the lungs and lymph nodes appears to be a 
      mechanism of PRRSV pathogenesis and might be an explanation for a dramatic 
      reduction in the number of alveolar macrophages and circulating lymphocytes and 
      monocytes in PRRSV-infected pigs.
FAU - Sirinarumitr, T
AU  - Sirinarumitr T
AD  - Department of Veterinary Pathology, Veterinary Medical Research Institute, 
      College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
FAU - Zhang, Y
AU  - Zhang Y
FAU - Kluge, J P
AU  - Kluge JP
FAU - Halbur, P G
AU  - Halbur PG
FAU - Paul, P S
AU  - Paul PS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Porcine Reproductive and Respiratory Syndrome/pathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Swine
MH  - United States
MH  - Virulence
EDAT- 1999/01/08 00:00
MHDA- 1999/01/08 00:01
CRDT- 1999/01/08 00:00
PHST- 1999/01/08 00:00 [pubmed]
PHST- 1999/01/08 00:01 [medline]
PHST- 1999/01/08 00:00 [entrez]
AID - 10.1099/0022-1317-79-12-2989 [doi]
PST - ppublish
SO  - J Gen Virol. 1998 Dec;79 ( Pt 12):2989-95. doi: 10.1099/0022-1317-79-12-2989.