PMID- 9883580
OWN - NLM
STAT- MEDLINE
DCOM- 19990210
LR  - 20071114
IS  - 0192-253X (Print)
IS  - 0192-253X (Linking)
VI  - 23
IP  - 4
DP  - 1998
TI  - Parental alleles of an imprinted mouse transgene replicate synchronously.
PG  - 275-84
AB  - Molecular features of imprinted genes include differences in expression, 
      methylation, and the timing of DNA replication between parental alleles. Whereas 
      methylation differences always seem to be associated with differences in 
      expression, differences in the timing of replication between parental homologs 
      are not always seen at imprinted loci. These observations raise the possibility 
      that differences in replication timing may not be an essential feature underlying 
      genomic imprinting. In this study, we examined the timing of replication of the 
      two alleles of the imprinted RSVIgmyc transgene in individual embryonic cells 
      using fluorescence in situ hybridization (FISH). The cis-acting signals for 
      RSVIgmyc imprinting are within RSVIgmyc itself. Thus, allele-specific differences 
      in replication, if they indeed govern RSVIgmyc imprinting, should be found in 
      RSVIgmyc sequences. We found that the parental alleles of RSVIgmyc, which exhibit 
      differences in methylation, replicated at the same time. Synchronous replication 
      was also seen in embryonic cells containing a modified version of RSVIgmyc that 
      exhibited parental allele differences in both methylation and expression. These 
      findings indicate that maintenance of expression and methylation differences 
      between alleles does not require a difference in replication timing. The 
      differences in replication timing of endogenous imprinted alleles detected by 
      FISH might therefore reflect structural differences between the two alleles that 
      could be a consequence of imprinting or, alternatively, could be unrelated to 
      imprinting.
FAU - Shuster, M
AU  - Shuster M
AD  - Department of Human Genetics, University of Pittsburgh, Graduate School of Public 
      Health, Pennsylvania, USA.
FAU - Dhar, M S
AU  - Dhar MS
FAU - Olins, A L
AU  - Olins AL
FAU - Olins, D E
AU  - Olins DE
FAU - Howell, C Y
AU  - Howell CY
FAU - Gollin, S M
AU  - Gollin SM
FAU - Chaillet, J R
AU  - Chaillet JR
LA  - eng
GR  - CA-09936/CA/NCI NIH HHS/United States
GR  - HD32940/HD/NICHD NIH HHS/United States
GR  - P30CA479049019/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Genet
JT  - Developmental genetics
JID - 7909963
SB  - IM
MH  - *Alleles
MH  - Animals
MH  - *DNA Replication
MH  - *Genomic Imprinting
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, Transgenic/*genetics
EDAT- 1999/01/12 03:01
MHDA- 2000/06/20 09:00
CRDT- 1999/01/12 03:01
PHST- 1999/01/12 03:01 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/01/12 03:01 [entrez]
AID - 10.1002/(SICI)1520-6408(1998)23:4<275::AID-DVG3>3.0.CO;2-# [pii]
AID - 10.1002/(SICI)1520-6408(1998)23:4<275::AID-DVG3>3.0.CO;2-# [doi]
PST - ppublish
SO  - Dev Genet. 1998;23(4):275-84. doi: 
      10.1002/(SICI)1520-6408(1998)23:4<275::AID-DVG3>3.0.CO;2-#.