PMID- 9884941
OWN - NLM
STAT- MEDLINE
DCOM- 19990331
LR  - 20161124
IS  - 0091-4916 (Print)
IS  - 0091-4916 (Linking)
VI  - 26
IP  - 4
DP  - 1998 Dec
TI  - Modified preparation and rapid quality control test for 
      technetium-99m-tetrofosmin.
PG  - 269-73
AB  - OBJECTIVE: The objectives of this study were to: modify the preparation of 
      99mTc-tetrofosmin by using twice the amount of 99mTcO4- recommended by the 
      manufacturer; evaluate the use of miniaturized rapid paper chromatography (MRPC) 
      for quality control (QC) testing; and determine the in vitro stability of the 
      modified preparation using MRPC. METHODS: Two preparations of 99mTc-tetrofosmin 
      were made: one with 4.4-8.8 GBq (120-240 mCi) and the other with 13.9-17.6 GBq 
      (380-480 mCi) 99mTcO4-, referred to as regular and modified preparations, 
      respectively. Routine QC tests were performed using MRPC and instant thin-layer 
      chromatography/silica-gel (ITLC/SG) systems. The preparations were injected into 
      58 patients. Planar and SPECT images of stress and rest studies were obtained. 
      The technical quality of the SPECT images was graded visually by four observers. 
      Heart-to-lung and heart-to-background ratios were calculated from the planar 
      images. RESULTS: The QC testing procedure took 4.18 +/- 0.15 min with MRPC and 54 
      +/- 5.3 min with ITLC/SG systems. The percent labeling efficiency, as determined 
      by both techniques, ranged from 95.6 +/- 1.6 to 97.2% +/- 0.8%. Both preparations 
      were stable up to 6 hr after reconstitution. There was no difference between the 
      cardiac-to-lung and cardiac-to-background ratios of the two preparations. 
      CONCLUSION: The results indicate that MRPC is a faster and effective 
      chromatographic technique for routine QC testing of 99mTc-tetrofosmin. Doubling 
      the amount of 99mTcO4- used in preparing 99mTc-tetrofosmin did not affect its in 
      vitro stability, its efficacious use in patients or the technical quality of the 
      images.
FAU - Patel, M
AU  - Patel M
AD  - Department of Nuclear Medicine, Kuwait Cancer Control Center, Kuwait City.
FAU - Owunwanne, A
AU  - Owunwanne A
FAU - Tuli, M
AU  - Tuli M
FAU - al-Za'abi, K
AU  - al-Za'abi K
FAU - al-Mohannadi, S
AU  - al-Mohannadi S
FAU - Sa'ad, M
AU  - Sa'ad M
FAU - Jahan, S
AU  - Jahan S
FAU - Jacob, A
AU  - Jacob A
FAU - al-Bunny, A
AU  - al-Bunny A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nucl Med Technol
JT  - Journal of nuclear medicine technology
JID - 0430303
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (Organotechnetium Compounds)
RN  - 0 (Radiopharmaceuticals)
RN  - 42FOP1YX93 (technetium tc-99m tetrofosmin)
SB  - IM
MH  - Chromatography, Paper
MH  - Chromatography, Thin Layer
MH  - Drug Stability
MH  - *Echo-Planar Imaging
MH  - Heart/*diagnostic imaging
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Lung/*diagnostic imaging
MH  - Organophosphorus Compounds/chemical synthesis/chemistry/*standards
MH  - Organotechnetium Compounds/chemical synthesis/chemistry/*standards
MH  - Quality Control
MH  - Radiopharmaceuticals/chemical synthesis/chemistry/*standards
MH  - *Tomography, Emission-Computed, Single-Photon
EDAT- 1999/01/13 00:00
MHDA- 1999/01/13 00:01
CRDT- 1999/01/13 00:00
PHST- 1999/01/13 00:00 [pubmed]
PHST- 1999/01/13 00:01 [medline]
PHST- 1999/01/13 00:00 [entrez]
PST - ppublish
SO  - J Nucl Med Technol. 1998 Dec;26(4):269-73.