PMID- 9886168
OWN - NLM
STAT- MEDLINE
DCOM- 19990316
LR  - 20150901
IS  - 1011-8934 (Print)
IS  - 1598-6357 (Electronic)
IS  - 1011-8934 (Linking)
VI  - 13
IP  - 6
DP  - 1998 Dec
TI  - Ultrastructural changes of the internal elastic lamina in experimental 
      hypercholesterolemic porcine coronary arteries.
PG  - 603-11
AB  - The internal elastic lamina (IEL) serves as a barrier for cells and 
      macromolecules between the intima and media in the vascular wall. We evaluated 
      the morphological changes and quantitative assessments of the IEL architecture in 
      the coronary circulation of pigs fed with a high cholesterol diet. Transmission 
      electron microscopy (TEM) analysis of the IEL from hypercholesterolemic coronary 
      arteries revealed fragmentation of the IEL associated with a decrease in the 
      thickness. Confocal microscopy and scanning electron microscopy (SEM) revealed an 
      altered pattern characterized by a large oval fenestration in the IEL of 
      hypercholesterolemic vessels. Morphometric analysis of confocal microscopy images 
      demonstrated that the IEL of cholesterol-fed animals were characterized by an 
      increase in the minor diameter of the fenestrae (2.16+/-0.04 microm vs 
      3.32+/-0.06 microm, p=0.003) and a decrease in the fenestrae density 
      (22,333+/-1,334/mm2 vs 17,552+/-931/mm2, p=0.015) compared to controls. The 
      percentage of the IEL area covered by the fenestrae correlated with the intimal 
      thickness (r=0.79, p=0.004). The immunoreactivity for matrix metalloproteinase-3 
      (MMP-3) increased in cholesterol-fed coronary arteries, predominantly in the 
      neointima. This study demonstrates experimental hypercholesterolemia induced 
      ultrastructural changes of the IEL in the coronary circulation. The IEL may play 
      an important role in the development of structural changes which characterize the 
      early phase of coronary atherosclerosis.
FAU - Kwon, H M
AU  - Kwon HM
AD  - Department of Internal Medicine, College of Medicine, Yonsei University, Seoul, 
      Korea. hmkwon@yumc.yonsei.ac.kr
FAU - Kim, D
AU  - Kim D
FAU - Hong, B K
AU  - Hong BK
FAU - Byun, K H
AU  - Byun KH
FAU - Oh, S H
AU  - Oh SH
FAU - Kna, J S
AU  - Kna JS
FAU - Kim, H S
AU  - Kim HS
FAU - Schwartz, R S
AU  - Schwartz RS
FAU - Lerman, A
AU  - Lerman A
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Korean Med Sci
JT  - Journal of Korean medical science
JID - 8703518
RN  - 0 (Lipoproteins, LDL)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Cholesterol/blood
MH  - Coronary Vessels/enzymology/*ultrastructure
MH  - Elastic Tissue/enzymology/*ultrastructure
MH  - Female
MH  - Hypercholesterolemia/blood/enzymology/*pathology
MH  - Immunohistochemistry
MH  - Lipoproteins, LDL/blood
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Microscopy, Confocal
MH  - Microscopy, Electron
MH  - Swine
PMC - PMC3054544
EDAT- 1999/01/14 00:00
MHDA- 1999/01/14 00:01
PMCR- 1998/12/01
CRDT- 1999/01/14 00:00
PHST- 1999/01/14 00:00 [pubmed]
PHST- 1999/01/14 00:01 [medline]
PHST- 1999/01/14 00:00 [entrez]
PHST- 1998/12/01 00:00 [pmc-release]
AID - 10.3346/jkms.1998.13.6.603 [doi]
PST - ppublish
SO  - J Korean Med Sci. 1998 Dec;13(6):603-11. doi: 10.3346/jkms.1998.13.6.603.