PMID- 9886550
OWN - NLM
STAT- MEDLINE
DCOM- 19990512
LR  - 20190512
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 13
IP  - 12
DP  - 1998 Dec
TI  - Cytokine secretion by human fetal membranes, decidua and placenta at term.
PG  - 3560-5
AB  - Cytokines such as monocyte chemotactic peptide-1 (MCP-1), interleukin-8 (IL-8), 
      RANTES (Regulated on Activation and Normally T-cells Expressed and presumably 
      Secreted) and interleukin-10 (IL-10) are thought to play pivotal roles in immune 
      recognition, acceptance of the fetal allograft, maintenance of pregnancy and 
      parturition. Their secretion and regulation within the third trimester uterus is, 
      however, less well defined. We therefore investigated the release of these 
      cytokines by third trimester amnion, chorion, placenta and decidua, and studied 
      the influence of prostaglandin E2 (PGE2) infusion on their release in a dynamic 
      placental cotyledon perfusion system. MCP-1 was released predominately by the 
      chorion (78.2 +/- 7.3 pg/mg wet tissue weight; mean +/- SEM), decidua (112.4 +/- 
      5.2 pg/mg) and placenta (101.8 +/- 5.0 pg/mg) with low amounts from the amnion 
      (1.3 +/- 0.4 pg/mg). High concentrations of IL-8 were released by the amnion 
      (39.9 +/- 5.3 pg/mg), chorion (52.8 +/- 1.9 pg/mg), decidua (42.2 +/- 1.5 pg/mg) 
      and placenta (45 +/- 1.3 pg/mg). Release of RANTES was not detectable from the 
      amnion but was detected in moderate amounts from the chorion (6.0 +/- 1.2 pg/mg), 
      decidua (15.2 +/- 1.4 pg/mg) and placenta (26.9 +/- 1.6 pg/mg). Low 
      concentrations of IL-10 were secreted by the chorion (6.8 +/- 0.8 pg/mg), decidua 
      (9.0 +/- 0.9 pg/mg) and placenta (3.3 +/- 0.3 pg/mg) with none detectable from 
      the amnion. MCP-1, IL-8, RANTES and IL-10 were all released by perfused placental 
      cotyledons. PGE2 stimulated release of MCP-1, IL-8 and IL-10 into the maternal 
      and of MCP-1 and IL-8 into the fetal circulation of the placenta but had no 
      effect on RANTES release. It is suggested that MCP-1 and IL-8 may be involved in 
      the inflammatory process of parturition and IL-10 in the protection of the fetal 
      allograft. In addition, PGE2 may have an important immunomodulatory role within 
      the uterus at term.
FAU - Denison, F C
AU  - Denison FC
AD  - Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for 
      Reproductive Biology, UK.
FAU - Kelly, R W
AU  - Kelly RW
FAU - Calder, A A
AU  - Calder AA
FAU - Riley, S C
AU  - Riley SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Cytokines)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Culture Techniques
MH  - Cytokines/*metabolism
MH  - Decidua/*metabolism
MH  - Dinoprostone/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Extraembryonic Membranes/*metabolism
MH  - Female
MH  - Humans
MH  - Placenta/*metabolism
MH  - Pregnancy
MH  - *Pregnancy Trimester, Third
EDAT- 1999/01/14 00:00
MHDA- 1999/01/14 00:01
CRDT- 1999/01/14 00:00
PHST- 1999/01/14 00:00 [pubmed]
PHST- 1999/01/14 00:01 [medline]
PHST- 1999/01/14 00:00 [entrez]
AID - 10.1093/humrep/13.12.3560 [doi]
PST - ppublish
SO  - Hum Reprod. 1998 Dec;13(12):3560-5. doi: 10.1093/humrep/13.12.3560.