PMID- 9886776 OWN - NLM STAT- MEDLINE DCOM- 19990225 LR - 20191102 IS - 0967-3849 (Print) IS - 0967-3849 (Linking) VI - 6 IP - 7 DP - 1998 Nov TI - Comparative analysis of crossover exchanges and chiasmata in Allium cepa x fistulosum after genomic in situ hybridization (GISH). PG - 567-74 AB - Genomic in situ hybridization (GISH) successfully differentiated homoeologous genomes in the inter-specific hybrid Allium cepa x fistulosum, thus allowing the detection of reciprocal crossover events as label exchanges in separating anaphase I chromosomes. Three of the eight chromosome pairs were positively identified by fluorescence in situ hybridization (FISH) to rDNA sequences. There was a general similarity of the GISH-based label exchange frequencies and metaphase I chiasma frequencies, but with a 20% deficit of chiasmata. Reasons for this apparent deficit are discussed. The locations of chiasmata and label exchanges are in broad agreement. FAU - Stevenson, M AU - Stevenson M AD - School of Biological Sciences, The University of Birmingham, Edgbaston, UK. FAU - Armstrong, S J AU - Armstrong SJ FAU - Ford-Lloyd, B V AU - Ford-Lloyd BV FAU - Jones, G H AU - Jones GH LA - eng PT - Comparative Study PT - Journal Article PL - Netherlands TA - Chromosome Res JT - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology JID - 9313452 SB - IM MH - Allium/*genetics/ultrastructure MH - Anaphase MH - Crossing Over, Genetic MH - Hybrid Cells MH - *In Situ Hybridization MH - In Situ Hybridization, Fluorescence MH - Meiosis MH - Metaphase EDAT- 1999/01/14 00:00 MHDA- 1999/01/14 00:01 CRDT- 1999/01/14 00:00 PHST- 1999/01/14 00:00 [pubmed] PHST- 1999/01/14 00:01 [medline] PHST- 1999/01/14 00:00 [entrez] AID - 10.1023/a:1009296826942 [doi] PST - ppublish SO - Chromosome Res. 1998 Nov;6(7):567-74. doi: 10.1023/a:1009296826942.