PMID- 9886860
OWN - NLM
STAT- MEDLINE
DCOM- 19990128
LR  - 20220309
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 140
IP  - 1
DP  - 1999 Jan
TI  - Ontogeny of estrogen receptor-beta expression in rat testis.
PG  - 478-83
AB  - The recently discovered estrogen receptor-beta (ERbeta) is expressed in rodent 
      and human testes. To obtain insight in the physiological role of ERbeta we have 
      investigated the cell type-specific expression pattern of ERbeta messenger RNA 
      (mRNA) and protein in the testis of rats of various ages by in situ hybridization 
      and immunohistochemistry. In fetal testes of rats 16 days postcoitum and testes 
      of 4-day-old animals, fetal germ cells (gonocytes) reveal the ERbeta mRNA in 
      their cytoplasm and the ERbeta protein in their nucleus. In testes of 11- and 
      15-day-old rats, ERbeta mRNA and protein were detected in Sertoli cells and type 
      A spermatogonia. No signal was found in other types of germ cells. In the adult 
      testes, expression of ERbeta mRNA as well as ERbeta protein was found in 
      pachytene spermatocytes from epithelial stages VII-XIV and in round spermatids 
      from stages I-VIII. Low ERbeta expression was observed in all type A 
      spermatogonia, including undifferentiated A spermatogonia, whereas no expression 
      was found in In and type B spermatogonia and early spermatocytes. At all ages, 
      Sertoli cells showed a weak hybridization signal as well as weak immunoreactivity 
      for ERbeta. In adult testes, no ERbeta mRNA or protein was detected in the 
      interstitial tissue, indicating that Leydig cells and peritubular myoid cells do 
      not express ERbeta. The expression of ERbeta in fetal and late male germ cells as 
      well as in Sertoli cells suggests that estrogens directly affect germ cells 
      during testicular development and spermatogenesis.
FAU - van Pelt, A M
AU  - van Pelt AM
AD  - Department of Cell Biology, Medical School, University of Utrecht, The 
      Netherlands. A.Pelt@pobox.accu.uu.nl
FAU - de Rooij, D G
AU  - de Rooij DG
FAU - van der Burg, B
AU  - van der Burg B
FAU - van der Saag, P T
AU  - van der Saag PT
FAU - Gustafsson, J A
AU  - Gustafsson JA
FAU - Kuiper, G G
AU  - Kuiper GG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Cell Nucleus/chemistry
MH  - Estrogen Receptor beta
MH  - Humans
MH  - In Situ Hybridization
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Estrogen/*biosynthesis/genetics
MH  - Spermatogonia/chemistry
MH  - Testis/embryology/*metabolism
EDAT- 1999/01/14 00:00
MHDA- 1999/01/14 00:01
CRDT- 1999/01/14 00:00
PHST- 1999/01/14 00:00 [pubmed]
PHST- 1999/01/14 00:01 [medline]
PHST- 1999/01/14 00:00 [entrez]
AID - 10.1210/endo.140.1.6438 [doi]
PST - ppublish
SO  - Endocrinology. 1999 Jan;140(1):478-83. doi: 10.1210/endo.140.1.6438.