PMID- 9888557
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20191024
IS  - 0743-5800 (Print)
IS  - 0743-5800 (Linking)
VI  - 24
IP  - 3-4
DP  - 1998 Aug-Nov
TI  - Cloning and expression of a novel tissue specific 17beta-hydroxysteroid 
      dehydrogenase.
PG  - 663-7
AB  - The 11beta-hydroxysteroid dehydrogenases (11betaHSD) modulate intracellular 
      glucocorticoid levels, with 11betaHSD1 converting cortisone to cortisol mainly in 
      the liver, and 11betaHSD2 performing the reverse reaction in sodium transporting 
      epithelia and placenta. We have attempted to expand the 11betaHSD subfamily by 
      isolating homologous cDNA's. Expressed Sequence Tag databases were screen with 
      segments of the 11betaHSD1 enzyme amino acid sequence and Pan1b identified as a 
      new member of the short chain alcohol dehydrogenase superfamily. Northern blot 
      analysis of total RNA from human tissues showed a single band at 1.9 kb and a 
      tissue specific pattern of expression with high levels in the liver, adrenal 
      carcinoma, lung and small intestine, and much lower levels in the kidney, heart 
      and placenta. Expression studies in a Chinese hamster ovary cell line (CHOP) 
      showed that Pan1b did not metabolize glucocorticoids. However, preliminary 
      studies on a range of substrates revealed that Pan1b acted as a dehydrogenase on 
      17beta-hydroxysteroids, although further kinetic analysis was confounded by large 
      amounts of endogenous oxidoreductase activity in CHOP cells. These studies 
      suggest the existence of a novel human 17betaHSD enzyme.
FAU - Li, K X
AU  - Li KX
AD  - Laboratory of Molecular Hypertension, Baker Medical Research Institute, 
      Melbourne, Australia.
FAU - Smith, R E
AU  - Smith RE
FAU - Krozowski, Z S
AU  - Krozowski ZS
LA  - eng
PT  - Journal Article
PL  - England
TA  - Endocr Res
JT  - Endocrine research
JID - 8408548
RN  - 0 (Isoenzymes)
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.- (HSD17B11 protein, human)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.1 (Alcohol Dehydrogenase)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - EC 1.2.- (Aldehyde Oxidoreductases)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - 17-Hydroxysteroid Dehydrogenases/*genetics/*metabolism
MH  - Alcohol Dehydrogenase/genetics
MH  - *Alcohol Oxidoreductases
MH  - *Aldehyde Oxidoreductases
MH  - Amino Acid Sequence/genetics
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Line
MH  - *Cloning, Molecular
MH  - Cricetinae
MH  - Databases as Topic
MH  - Expressed Sequence Tags
MH  - Female
MH  - Humans
MH  - Hydroxysteroid Dehydrogenases/genetics
MH  - Isoenzymes/*genetics/*metabolism
MH  - Mesocricetus
MH  - Multigene Family
MH  - Ovary/cytology/metabolism
MH  - Tissue Distribution
EDAT- 1999/01/15 00:00
MHDA- 1999/01/15 00:01
CRDT- 1999/01/15 00:00
PHST- 1999/01/15 00:00 [pubmed]
PHST- 1999/01/15 00:01 [medline]
PHST- 1999/01/15 00:00 [entrez]
AID - 10.3109/07435809809032667 [doi]
PST - ppublish
SO  - Endocr Res. 1998 Aug-Nov;24(3-4):663-7. doi: 10.3109/07435809809032667.