PMID- 9888867
OWN - NLM
STAT- MEDLINE
DCOM- 19990420
LR  - 20190831
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 19
IP  - 1
DP  - 1999 Jan
TI  - Dietary omega-3, omega-6, and omega-9 unsaturated fatty acids and growth factor 
      and cytokine gene expression in unstimulated and stimulated monocytes. A 
      randomized volunteer study.
PG  - 59-66
AB  - Dietary omega-3 fatty acids retard coronary atherosclerosis. Previously, we 
      demonstrated that dietary omega-3 fatty acids reduce platelet-derived growth 
      factor (PDGF)-A and PDGF-B mRNA levels in unstimulated, human mononuclear cells 
      (MNCs). In a randomized, investigator-blinded intervention trial, we have now 
      compared the effect of ingestion of 7 g/d omega-3, omega-6, or omega-9 fatty 
      acids for 4 weeks versus no dietary intervention on PDGF-A, PDGF-B, heparin-bound 
      epidermal growth factor (HB-EGF), monocyte chemoattractant protein-1 (MCP-1), and 
      interleukin-10 gene expression in unstimulated MNCs and in monocytes that were 
      adherence-activated ex vivo in a total of 28 volunteers. In unstimulated MNCs, 
      mRNA steady-state levels of PDGF-A, PDGF-B, and MCP-1 were reduced by 25+/-10%, 
      31+/-13%, and 40+/-14%, respectively, after omega-3 fatty acid ingestion, as 
      assessed by quantitative polymerase chain reaction (all P<0.05). In monocytes 
      that were adherence-activated ex vivo for 4 and 20 hours, mRNA steady-state 
      levels of PDGF-A, PDGF-B, and MCP-1 were reduced by 25+/-13%, 20+/-15%, and 
      30+/-8%, respectively (all P<0.05). Interleukin-10 and HB-EGF mRNA steady-state 
      levels were not influenced by omega-3 fatty acid ingestion. Expression of all 
      respective mRNAs in control volunteers or in those ingesting omega-6 or omega-9 
      fatty acids were not altered. We conclude that human gene expression for PDGF-A, 
      PDGF-B, and MCP-1, factors thought relevant to atherosclerosis, is constitutive, 
      is constant, and can be reduced only by dietary omega-3 fatty acids in 
      unstimulated and adherence-activated monocytes.
FAU - Baumann, K H
AU  - Baumann KH
AD  - Medizinische Klinik, Klinikum Innenstadt, University of Munich, Munich, Germany.
FAU - Hessel, F
AU  - Hessel F
FAU - Larass, I
AU  - Larass I
FAU - Muller, T
AU  - Muller T
FAU - Angerer, P
AU  - Angerer P
FAU - Kiefl, R
AU  - Kiefl R
FAU - von Schacky, C
AU  - von Schacky C
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Dietary Fats, Unsaturated)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Fatty Acids, Omega-6)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Growth Substances)
RN  - 0 (HBEGF protein, human)
RN  - 0 (Heparin-binding EGF-like Growth Factor)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (RNA, Messenger)
RN  - 0 (platelet-derived growth factor A)
RN  - 130068-27-8 (Interleukin-10)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics
MH  - Cytokines/*genetics
MH  - Dietary Fats, Unsaturated/administration & dosage/*pharmacology
MH  - Epidermal Growth Factor/genetics
MH  - Fatty Acids, Omega-3/administration & dosage/pharmacology
MH  - Fatty Acids, Omega-6
MH  - Fatty Acids, Unsaturated/administration & dosage/*pharmacology
MH  - *Gene Expression
MH  - Growth Substances/*genetics
MH  - Heparin-binding EGF-like Growth Factor
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins
MH  - Interleukin-10/genetics
MH  - Male
MH  - Monocytes/*metabolism
MH  - Platelet-Derived Growth Factor/genetics
MH  - Proto-Oncogene Proteins/genetics
MH  - Proto-Oncogene Proteins c-sis
MH  - RNA, Messenger/analysis/metabolism
EDAT- 1999/01/15 00:00
MHDA- 1999/01/15 00:01
CRDT- 1999/01/15 00:00
PHST- 1999/01/15 00:00 [pubmed]
PHST- 1999/01/15 00:01 [medline]
PHST- 1999/01/15 00:00 [entrez]
AID - 10.1161/01.atv.19.1.59 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 1999 Jan;19(1):59-66. doi: 10.1161/01.atv.19.1.59.