PMID- 9888870
OWN - NLM
STAT- MEDLINE
DCOM- 19990420
LR  - 20190831
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 19
IP  - 1
DP  - 1999 Jan
TI  - Studies on the histogenesis of myxomatous tissue of human coronary lesions.
PG  - 83-97
AB  - Myxomatous tissue is a characteristic component of human coronary artery lesions, 
      found more often in restenotic lesions. It represents a bulky accumulation of 
      stellate-shaped cells of unknown histogenesis that are embedded in a loose 
      stroma. We analyzed 64 atherectomy specimens containing substantial amounts of 
      myxomatous tissue by using immunohistochemistry, in situ hybridization, and 
      electron microscopy techniques. Stellate cells represented a heterogeneous 
      population, sharing features of smooth muscle cells (SMCs), macrophages, as well 
      as antigen-presenting dendritic cells. Like quiescent medial SMCs, the stellate 
      cells in all specimens expressed high levels of SM alpha-actin message and 
      protein and showed heterogeneity with respect to heavy-chain myosin, SM22, 
      desmin, and vimentin. Ultrastructurally, stellate cells resembled SMCs, with some 
      peculiarities that distinguish them from both differentiated and dedifferentiated 
      SMCs. In contrast to quiescent SMCs, the stellate cells expressed high levels of 
      acidic fibroblast growth factor mRNA and protein similar to cells of 
      monocyte/macrophage lineage. However, stellate cells did not express the marker 
      of mature macrophages, HAM56, and were heterogeneous with respect to CD68. 
      Moreover, unlike SMCs, the stellate cells bore some of the major phenotypic 
      markers of dendritic cells: they were S100-positive and showed various reactivity 
      with respect to CD1a and human leukocyte antigen (HLA)-DR. Invasion of myxomatous 
      tissue with CD45RO-positive T lymphocytes was correlated with strong expression 
      of CD1a in these specimens. Stellate cells also expressed a pericyte marker, 
      high-molecular-weight melanoma-associated antigen. We conclude that stellate 
      cells of myxomatous tissue represent a specific phenotype of mesenchymal cells 
      (possibly pericytes), which is activated to express some markers of 
      antigen-presenting cells. These findings suggest involvement of the stellate 
      cells in a local immune response.
FAU - Tjurmin, A V
AU  - Tjurmin AV
AD  - Department of Experimental Pathology, J.H. Holland Laboratory, American Red 
      Cross, Rockville, Md, USA.
FAU - Ananyeva, N M
AU  - Ananyeva NM
FAU - Smith, E P
AU  - Smith EP
FAU - Gao, Y
AU  - Gao Y
FAU - Hong, M K
AU  - Hong MK
FAU - Leon, M B
AU  - Leon MB
FAU - Haudenschild, C C
AU  - Haudenschild CC
LA  - eng
GR  - HL 54246/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Actins)
RN  - 0 (Proteoglycans)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 104781-85-3 (Fibroblast Growth Factor 1)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Actins/analysis/genetics
MH  - Aged
MH  - Atherectomy
MH  - Cell Nucleus/pathology
MH  - Collagen/analysis
MH  - Coronary Disease/*pathology/surgery
MH  - Coronary Vessels/*pathology
MH  - Cytoplasm/pathology
MH  - Dendritic Cells/immunology/pathology
MH  - Extracellular Matrix/pathology
MH  - Fibroblast Growth Factor 1
MH  - Fibroblast Growth Factor 2/analysis
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Microscopy, Electron
MH  - Middle Aged
MH  - Myxoma/*pathology
MH  - Proteoglycans/analysis
MH  - Stromal Cells/pathology
EDAT- 1999/01/15 00:00
MHDA- 1999/01/15 00:01
CRDT- 1999/01/15 00:00
PHST- 1999/01/15 00:00 [pubmed]
PHST- 1999/01/15 00:01 [medline]
PHST- 1999/01/15 00:00 [entrez]
AID - 10.1161/01.atv.19.1.83 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 1999 Jan;19(1):83-97. doi: 10.1161/01.atv.19.1.83.