PMID- 9889772
OWN - NLM
STAT- MEDLINE
DCOM- 19990129
LR  - 20161020
IS  - 0869-8031 (Print)
IS  - 0869-8031 (Linking)
VI  - 38
IP  - 6
DP  - 1998 Nov-Dec
TI  - [Registration of stable aberrations in peripheral blood lymphocytes using 
      G-differential chromosome staining and fluorescence in situ hybridization 
      methods].
PG  - 793-9
AB  - G-banding analysis and fluorescence in situ hybridization (FISH) for whole 
      chromosomes 1, 2 and 4 were applied in comparative assay for frequency of stable 
      chromosome aberrations in 37 individuals with previous exposure to radiation (15 
      clean-up workers/liquidators of Chernobyl nuclear power plant accident and 22 
      residents of radiocontaminated areas) and in 18 individuals of a reference group. 
      In G-banding analysis of stable aberrations, we used classification of Ohtaki K. 
      et al., 1992, in compliance with ISCN, 1985. FISH-assay for translocations is 
      performed in accordance with classification of Tucker J.D. et al., 1995. 
      Comparison of the results reveals statistical trustworthy correlation between the 
      two assays. The results point out that FISH for translocations in as few as three 
      chromosomes, when combined with screening of numerous metaphases, provides 
      sensitivity comparable with that provided by G-banding which covers the whole 
      genome.
FAU - Obukhova, T N
AU  - Obukhova TN
AD  - National Research Center for Hematology, Russian Academy of Medical Sciences, 
      Moscow.
FAU - Domracheva, E V
AU  - Domracheva EV
LA  - rus
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
TT  - Registratsiia stabil'nykh aberratsii v limfotsitakh perifericheskoi krovi 
      metodami G-differentsial'nogo okrashivaniia khromosom i fliuorestsentnoi in situ 
      gibridizatsii.
PL  - Russia (Federation)
TA  - Radiats Biol Radioecol
JT  - Radiatsionnaia biologiia, radioecologiia
JID - 9317212
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Air Pollution, Radioactive
MH  - Cells, Cultured
MH  - Child
MH  - *Chromosome Aberrations
MH  - *Chromosome Banding
MH  - Chromosomes/*radiation effects
MH  - Chromosomes, Human, Pair 1/genetics/radiation effects
MH  - Chromosomes, Human, Pair 2/genetics/radiation effects
MH  - Chromosomes, Human, Pair 4/genetics/radiation effects
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphocytes/cytology/radiation effects
MH  - Male
MH  - Metaphase/genetics
MH  - Middle Aged
MH  - Models, Theoretical
MH  - *Power Plants
MH  - *Radioactive Hazard Release
MH  - Translocation, Genetic
MH  - Ukraine
EDAT- 1999/01/16 00:00
MHDA- 1999/01/16 00:01
CRDT- 1999/01/16 00:00
PHST- 1999/01/16 00:00 [pubmed]
PHST- 1999/01/16 00:01 [medline]
PHST- 1999/01/16 00:00 [entrez]
PST - ppublish
SO  - Radiats Biol Radioecol. 1998 Nov-Dec;38(6):793-9.