PMID- 9890417
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20131121
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 57
IP  - 1
DP  - 1999 Jan
TI  - A human cell line constitutively expressing HIV-1 Gag and Gag-Pol gene products.
PG  - 17-24
AB  - A human cell line constitutively expressing the HIV-1 gag and pol genes products 
      was established. The cell line was established by stably transfecting 293 cells 
      with a plasmid construct that expresses the HIV Gag and Pol and can confer the 
      transfectants resistant to mycophenolic acid. Particles generated from transient 
      expression of the plasmid construct were noninfectious when pseudotyped with HIV 
      envelope or with amphotropic murine leukemia virus envelope proteins. However, 
      virus-like Gag particles produced by the stable cell line were appropriately 
      processed, exhibited a wild-type retrovirus particle density, and possessed 
      significant reverse-transcriptase (RT) activities. Continuous passage of the cell 
      line either in the presence or absence of mycophenolic acid had no major effects 
      on the Gag processing efficiency, particle assembly, or RT activity release. It 
      was also demonstrated that the proteolytic processing of the virus-like particles 
      released from the cell line was inhibited by an HIV protease inhibitor, 
      saquinavir. The establishment of a stable cell line producing noninfectious but 
      proteolytically processed HIV Gag particles offers a safe, convenient tool for 
      biochemical and immunological analysis of virus-like particle assembly and is 
      very useful for the development of anti-HIV protease drugs.
FAU - Wang, C T
AU  - Wang CT
AD  - Institute of Clinical Medicine, National Yang-Ming University, Department of 
      Medical Research, Veterans General Hospital-Taipei, Taiwan. ctwang@vghtpe.gov.tw
FAU - Li, J J
AU  - Li JJ
FAU - Lai, H Y
AU  - Lai HY
FAU - Hu, B S
AU  - Hu BS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Fusion Proteins, gag-pol)
RN  - 0 (Gene Products, gag)
RN  - 0 (HIV Protease Inhibitors)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.7.49 (HIV Reverse Transcriptase)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
RN  - L3JE09KZ2F (Saquinavir)
SB  - IM
MH  - Blotting, Western
MH  - Cell Line/drug effects/*metabolism/virology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fusion Proteins, gag-pol/*metabolism
MH  - Gene Products, gag/*metabolism
MH  - HIV Protease Inhibitors/pharmacology
MH  - HIV Reverse Transcriptase/analysis
MH  - HIV-1/drug effects/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - RNA-Directed DNA Polymerase
MH  - Saquinavir/pharmacology
MH  - Transformation, Genetic
MH  - Viral Proteins/biosynthesis/*genetics
EDAT- 1999/01/16 03:13
MHDA- 2000/06/20 09:00
CRDT- 1999/01/16 03:13
PHST- 1999/01/16 03:13 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/01/16 03:13 [entrez]
AID - 10.1002/(SICI)1096-9071(199901)57:1<17::AID-JMV3>3.0.CO;2-9 [pii]
PST - ppublish
SO  - J Med Virol. 1999 Jan;57(1):17-24.