PMID- 9891502
OWN - NLM
STAT- MEDLINE
DCOM- 19990211
LR  - 20091119
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 18
IP  - 6A
DP  - 1998 Nov-Dec
TI  - Odontogenic myxomas are not associated with activating mutations of the Gs alpha 
      gene.
PG  - 4415-7
AB  - BACKGROUND: Myxoma is a rare bone tumor of the mandible and maxillary sinus whose 
      etiology and underlying molecular mechanisms remain unknown. Mutations that 
      inhibit the GTPase activity of the a subunit of the stimulating G protein (Gsa) 
      have been demonstrated in the myocardium of patients with McCune-Albright 
      syndrome. The histopathological similarities shared by cardiac and jaw myxomas 
      coupled with the paucity of reported candidate genes involved in jaw tumor 
      pathogenesis, prompted us to investigate for the presence of gsp mutations in 23 
      sporadic jaw myxomas. MATERIALS AND METHODS: We used the polymerase chain 
      reaction (PCR) to amplify the appropriate genomic fragments, followed by 
      denaturing gradient gel electrophoresis (DGGE) analysis. RESULTS: No gsp 
      mutations could be demonstrated in any of tumors analyzed, while the technique 
      has a proven capability to detect these specific mutations. CONCLUSIONS: We 
      conclude that mutations of the Gs alpha gene rarely, if ever, are associated with 
      sporadic jaw myxomas tumorigenesis.
FAU - Boson, W L
AU  - Boson WL
AD  - Department of Pharmacology, Instituto de Ciencias Biologicas, Belo Horizonte, 
      Brazil.
FAU - Gomez, R S
AU  - Gomez RS
FAU - Araujo, L
AU  - Araujo L
FAU - Kalapothakis, E
AU  - Kalapothakis E
FAU - Friedman, E
AU  - Friedman E
FAU - De Marco, L
AU  - De Marco L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (DNA, Neoplasm)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gs)
SB  - IM
MH  - Biopsy
MH  - DNA, Neoplasm/genetics
MH  - GTP Phosphohydrolases/genetics/metabolism
MH  - GTP-Binding Protein alpha Subunits, Gs/*genetics/metabolism
MH  - Humans
MH  - Jaw Neoplasms/*genetics/pathology
MH  - Mutation
MH  - Myxoma/*genetics/pathology
MH  - Odontogenic Tumors/*genetics/pathology
MH  - Polymerase Chain Reaction
EDAT- 1999/01/19 00:00
MHDA- 1999/01/19 00:01
CRDT- 1999/01/19 00:00
PHST- 1999/01/19 00:00 [pubmed]
PHST- 1999/01/19 00:01 [medline]
PHST- 1999/01/19 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1998 Nov-Dec;18(6A):4415-7.