PMID- 9891847
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20191024
IS  - 1039-9712 (Print)
IS  - 1039-9712 (Linking)
VI  - 46
IP  - 6
DP  - 1998 Dec
TI  - Hexokinase, glucose-6-phosphate dehydrogenase and antioxidant enzymes in diabetic 
      reticulocytes: effects of insulin and vanadate.
PG  - 1145-52
AB  - Experimentally induced diabetic rats were treated separately with insulin and 
      vanadate. The activities of hexokinase (HK) and glucose-6-phosphate dehydrogenase 
      (G-6PDH) were increased in reticulocyte hemolysate isolated from the diabetic 
      rats and were restored to normal levels by insulin. The restoration was not 
      detected in vanadate treated diabetic animals. The enzymes of glutathione 
      metabolism namely glutathione peroxidase (GPx), glutathione reductase (GR) and 
      glutathione-s-transferase (GST) exhibited increases in their activities with 
      diabetes and were restored to almost control values by insulin treatment. 
      Vanadate given to diabetic animals further increased GPx, and GST. The level of 
      superoxide dismutase(SOD) decreased in the reticulocytes of diabetic rats and 
      catalase (CAT) was unchanged. Both CAT and SOD had normal values when the 
      diabetic rats were treated with insulin and vanadate. It is proposed that 
      vanadate may cause an increase in the activity of GR which may stimulate glucose 
      transporters and glucose metabolism.
FAU - Gupta, B L
AU  - Gupta BL
AD  - Hormone and Drug Research Laboratory, School of Life Sciences, Jawaharlal Nehru 
      University, New Delhi, India.
FAU - Baquer, N Z
AU  - Baquer NZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem Mol Biol Int
JT  - Biochemistry and molecular biology international
JID - 9306673
RN  - 0 (Insulin)
RN  - 3WHH0066W5 (Vanadates)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.8.1.7 (Glutathione Reductase)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Catalase/blood
MH  - Diabetes Mellitus, Experimental/blood/*enzymology
MH  - Glucosephosphate Dehydrogenase/*blood
MH  - Glutathione Peroxidase/blood
MH  - Glutathione Reductase/blood
MH  - Glutathione Transferase/blood
MH  - Hexokinase/*blood
MH  - Insulin/*pharmacology
MH  - Male
MH  - Peroxidases/*blood
MH  - Rats
MH  - Rats, Wistar
MH  - Reference Values
MH  - Reticulocytes/drug effects/*enzymology
MH  - Superoxide Dismutase/blood
MH  - Vanadates/*pharmacology
EDAT- 1999/01/19 00:00
MHDA- 1999/01/19 00:01
CRDT- 1999/01/19 00:00
PHST- 1999/01/19 00:00 [pubmed]
PHST- 1999/01/19 00:01 [medline]
PHST- 1999/01/19 00:00 [entrez]
AID - 10.1080/15216549800204702 [doi]
PST - ppublish
SO  - Biochem Mol Biol Int. 1998 Dec;46(6):1145-52. doi: 10.1080/15216549800204702.