PMID- 9892992
OWN - NLM
STAT- MEDLINE
DCOM- 19990219
LR  - 20191102
IS  - 0361-090X (Print)
IS  - 0361-090X (Linking)
VI  - 23
IP  - 1
DP  - 1999
TI  - Preparation of a diagnostic antigen of human melanoma based on lymphocyte 
      activation as measured by intracellular fluorescein fluorescence polarization.
PG  - 64-71
AB  - The intracellular fluorescein fluorescence polarization (IFFP) test indicates 
      that peripheral blood lymphocytes (PBL) of cancer patients display stimulatory 
      sensitivity to a short incubation with specific tumor protein extracts. In this 
      work, a human lymphocyte activation melanoma antigen (LAMA) was purified from 
      supernatant of a human melanoma cell line (L1M1), which could specifically 
      stimulate lymphocytes of melanoma patients. The results showed a significant 
      stimulation of lymphocytes from healthy donors after incubation with 
      phytohaemagglutinin (PHA), while no stimulation was observed after incubation 
      with LAMA. On the other hand, lymphocytes from melanoma patients showed a 
      significant stimulation with LAMA, while generally showing minor or no 
      stimulation with PHA. Melanoma specificity of LAMA was demonstrated by no 
      response in lymphocytes from patients of lung, colon, or breast cancer. The 
      purified fraction is therefore considered to be a shared tissue-specific antigen 
      which may be useful in immunodiagnosis and immunotherapy of melanoma.
FAU - Avtalion, N
AU  - Avtalion N
AD  - The Jerome Schottenstein Cellscan Center for Early Detection of Cancer, Physics 
      Department, Bar-Ilan University, Ramat-Gan, Israel.
FAU - Avtalion, R
AU  - Avtalion R
FAU - Tirosh, R
AU  - Tirosh R
FAU - Sheinberg, A
AU  - Sheinberg A
FAU - Weinreb, A
AU  - Weinreb A
FAU - Avinoach, I
AU  - Avinoach I
FAU - Deutsch, M
AU  - Deutsch M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Cancer Detect Prev
JT  - Cancer detection and prevention
JID - 7704778
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Melanoma-Specific Antigens)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Phytohemagglutinins)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - *Antigens, Neoplasm/immunology/isolation & purification
MH  - Biomarkers, Tumor
MH  - Chromatography, DEAE-Cellulose
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fluorescein
MH  - Fluorescence Polarization
MH  - Humans
MH  - *Lymphocyte Activation
MH  - Lymphocytes/*immunology
MH  - Melanoma/chemistry/*diagnosis/immunology
MH  - Melanoma-Specific Antigens
MH  - *Neoplasm Proteins/immunology/isolation & purification
MH  - Phytohemagglutinins
MH  - Tumor Cells, Cultured
EDAT- 1999/01/20 00:00
MHDA- 1999/01/20 00:01
CRDT- 1999/01/20 00:00
PHST- 1999/01/20 00:00 [pubmed]
PHST- 1999/01/20 00:01 [medline]
PHST- 1999/01/20 00:00 [entrez]
AID - 10.1046/j.1525-1500.1999.09901.x [doi]
PST - ppublish
SO  - Cancer Detect Prev. 1999;23(1):64-71. doi: 10.1046/j.1525-1500.1999.09901.x.