PMID- 9893060
OWN - NLM
STAT- MEDLINE
DCOM- 19990222
LR  - 20061115
IS  - 0884-0431 (Print)
IS  - 0884-0431 (Linking)
VI  - 14
IP  - 1
DP  - 1999 Jan
TI  - Studies of the murine homolog of the multiple endocrine neoplasia type 1 (MEN1) 
      gene, men1.
PG  - 3-10
AB  - The murine homolog of the multiple endocrine neoplasia type 1 (MEN1) gene (men1), 
      which in humans is associated with tumors of the parathyroids, pancreas, and 
      pituitary, has been characterized by isolating 27 clones from a mouse embryonic 
      stem cell cDNA library. The insert sizes ranged from 600-2500 bp, and sequence 
      analysis identified a 1833 bp open reading frame encoding a 611 amino acid 
      protein. In addition, two clones contained an unspliced intron 1, and another two 
      clones contained 20-29 bp of an upstream sequence, which suggested the presence 
      of an alternate exon 1. This was supported by an analysis of the homologous human 
      sequence. The mouse and human coding regions had 89% and 96% identity of the 
      nucleotide and amino acid sequences, respectively. Investigation of clones 
      isolated from a 129ola mouse genomic library, revealed the men1 gene to consist 
      of 10 exons that spanned approximately 6 kb. Northern blot analysis demonstrated 
      the ubiquitous expression of 2.9 kb and 3. 4 kb transcripts in mouse adult 
      tissues and embryos from 7 days. DNA sequence analysis of the larger 3.4 kb 
      transcript revealed it to result from a retention of intron 1. In situ 
      hybridization confirmed an early ubiquitous expression in whole mount mouse 
      embryos and adult tissues, but in the latter, different levels of cellular 
      expression were observed, e.g., men1 expression was higher in testicular Sertoli 
      cells than in germ cells. Thus, the mouse men1 gene and the basis of alternative 
      transcripts have been defined, and these will help to facilitate studies of a 
      mouse model.
FAU - Bassett, J H
AU  - Bassett JH
AD  - MRC Molecular Endocrinology Group, MRC Clinical Sciences Center, Imperial College 
      School of Medicine, Hammersmith Hospital, London, United Kingdom.
FAU - Rashbass, P
AU  - Rashbass P
FAU - Harding, B
AU  - Harding B
FAU - Forbes, S A
AU  - Forbes SA
FAU - Pannett, A A
AU  - Pannett AA
FAU - Thakker, R V
AU  - Thakker RV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - DNA, Complementary/isolation & purification
MH  - *Gene Library
MH  - Genome
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multiple Endocrine Neoplasia Type 1/*genetics
MH  - Sequence Homology, Nucleic Acid
EDAT- 1999/01/20 00:00
MHDA- 1999/01/20 00:01
CRDT- 1999/01/20 00:00
PHST- 1999/01/20 00:00 [pubmed]
PHST- 1999/01/20 00:01 [medline]
PHST- 1999/01/20 00:00 [entrez]
AID - 10.1359/jbmr.1999.14.1.3 [doi]
PST - ppublish
SO  - J Bone Miner Res. 1999 Jan;14(1):3-10. doi: 10.1359/jbmr.1999.14.1.3.