PMID- 9895334
OWN - NLM
STAT- MEDLINE
DCOM- 19990128
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 45
IP  - 1
DP  - 1999 Jan
TI  - Identification of MEN1 mutations in sporadic enteropancreatic neuroendocrine 
      tumors by analysis of paraffin-embedded tissue.
PG  - 29-34
AB  - Gastrinomas and other gastrointestinal neuroendocrine tumors may occur 
      sporadically or as part of the inherited syndrome multiple endocrine neoplasia 
      type 1 (MEN1). Mutations in the recently identified MEN1 gene have been described 
      in sporadic gastrinomas and insulinomas. This study describes techniques used to 
      identify mutations in the MEN1 gene in DNA extracted from paraffin-preserved 
      tissue. Two novel mutations are identified in the MEN1 gene from nine archived 
      paraffin-embedded neuroendocrine tumors, demonstrating that retrospective genetic 
      analysis can be used to identify mutations in the MEN1 gene from preserved 
      tissue. Conditions are provided by which paraffin-embedded tissue can be used as 
      a source of genetic material for sequence information of sufficient quality for 
      mutational studies of the MEN1 gene. It should also be possible to apply this 
      retrospective genetic analysis of paraffin-embedded tissue to other disease 
      models.
FAU - Mailman, M D
AU  - Mailman MD
AD  - Department of Pathology, The Ohio State University College of Medicine, Columbus 
      43210, USA.
FAU - Muscarella, P
AU  - Muscarella P
FAU - Schirmer, W J
AU  - Schirmer WJ
FAU - Ellison, E C
AU  - Ellison EC
FAU - O'Dorisio, T M
AU  - O'Dorisio TM
FAU - Prior, T W
AU  - Prior TW
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (DNA, Neoplasm)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
SB  - IM
MH  - DNA, Neoplasm/analysis
MH  - Duodenal Neoplasms/genetics/pathology
MH  - Gastrinoma/genetics/pathology
MH  - Gastrointestinal Neoplasms/*genetics/pathology
MH  - Heterozygote
MH  - Humans
MH  - Insulinoma/genetics/pathology
MH  - Liver Neoplasms/genetics/pathology
MH  - Multiple Endocrine Neoplasia Type 1/*genetics
MH  - Mutation
MH  - Neoplasm Proteins/*genetics
MH  - Neuroendocrine Tumors/*genetics/pathology
MH  - Pancreatic Neoplasms/*genetics/pathology
MH  - Paraffin Embedding
MH  - *Proto-Oncogene Proteins
MH  - Stomach Neoplasms/genetics/pathology
EDAT- 1999/01/23 00:00
MHDA- 1999/01/23 00:01
CRDT- 1999/01/23 00:00
PHST- 1999/01/23 00:00 [pubmed]
PHST- 1999/01/23 00:01 [medline]
PHST- 1999/01/23 00:00 [entrez]
PST - ppublish
SO  - Clin Chem. 1999 Jan;45(1):29-34.