PMID- 9895344
OWN - NLM
STAT- MEDLINE
DCOM- 19990128
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 45
IP  - 1
DP  - 1999 Jan
TI  - Improved extraction procedure and RIA for determination of arginine8-vasopressin 
      in plasma: role of premeasurement sample treatment and reference values in 
      children.
PG  - 98-103
AB  - We optimized an RIA for measurement of arginine8-vasopressin (AVP) in plasma by 
      use of 100-mg Isolute C18 columns for extraction, addition of a preincubation 
      step, and use of maximal dilution of a commercially available antiserum. The 
      detection limit was 0.06 ng/L when 0.5 mL of acidified plasma was extracted. The 
      within- and between-run CVs (n = 16) at physiological concentrations were 5.8-10. 
      2% and 6.5-11.7%, respectively. Prolonged storage of blood at 25 degreesC, but 
      not at 4 degreesC, led to a significant increase in measured plasma AVP 
      concentrations. When plasma samples were prepared at several centrifugation 
      speeds, plasma AVP was significantly correlated with the platelet count (r = 
      0.899; P <0. 001). This emphasizes the need for careful sample preparation to 
      avoid contamination of plasma with platelet-bound AVP. Basal plasma AVP in 203 
      children and adolescents (105 males and 98 females; ages, 1 day to 18 years) 
      averaged 1.1 +/- 0.6 ng/L. There was no significant difference between males and 
      females and no correlation with age. In 16 healthy adult controls, plasma AVP 
      averaged 1.0 +/- 0.5 ng/L.
FAU - Kluge, M
AU  - Kluge M
AD  - Department of Pediatrics, University of Vienna, Wahringer Gurtel 18-20, A-1090 
      Vienna, Austria.
FAU - Riedl, S
AU  - Riedl S
FAU - Erhart-Hofmann, B
AU  - Erhart-Hofmann B
FAU - Hartmann, J
AU  - Hartmann J
FAU - Waldhauser, F
AU  - Waldhauser F
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (Immune Sera)
RN  - 0 (Renal Agents)
RN  - 113-79-1 (Arginine Vasopressin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Arginine Vasopressin/*blood/immunology
MH  - Blood Specimen Collection
MH  - Calibration
MH  - Centrifugation
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Immune Sera
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Platelet Count
MH  - Radioimmunoassay
MH  - Reference Values
MH  - Renal Agents/*blood/immunology
MH  - Sensitivity and Specificity
EDAT- 1999/01/23 00:00
MHDA- 1999/01/23 00:01
CRDT- 1999/01/23 00:00
PHST- 1999/01/23 00:00 [pubmed]
PHST- 1999/01/23 00:01 [medline]
PHST- 1999/01/23 00:00 [entrez]
PST - ppublish
SO  - Clin Chem. 1999 Jan;45(1):98-103.