PMID- 9914164
OWN - NLM
STAT- MEDLINE
DCOM- 19990720
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 4)
DP  - 1999 Feb
TI  - Chromosomes exhibit preferential positioning in nuclei of quiescent human cells.
PG  - 525-35
AB  - The relative spatial positioning of chromosomes 7, 8, 16, X and Y was examined in 
      nuclei of quiescent (noncycling) diploid and triploid human fibroblasts using 
      fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes and 
      digital imaging. In quiescent diploid cells, interhomolog distances and 
      chromosome homolog position maps revealed a nonrandom, preferential topology for 
      chromosomes 7, 8 and 16, whereas chromosome X approximated a more random 
      distribution. Variations in the orientation of nuclei on the culture substratum 
      tended to hinder detection of an ordered chromosome topology at interphase by 
      biasing homolog position maps towards random distributions. Using two chromosome 
      X homologs as reference points in triploid cells (karyotype = 69, XXY), the 
      intranuclear location of chromosome Y was found to be predictable within 
      remarkably narrow spatial limits. Dual-FISH with various combinations of 
      chromosome-specific DNA probes and contrasting fluorochromes was used to identify 
      adjacent chromosomes in mitotic rosettes and test whether they are similarly 
      positioned in interphase nuclei. From among the combinations tested, chromosomes 
      8 and 11 were found to be closely apposed in most mitotic rosettes and interphase 
      nuclei. Overall, results suggest the existence of an ordered interphase 
      chromosome topology in quiescent human cells in which at least some chromosome 
      homologs exhibit a preferred relative intranuclear location that may correspond 
      to the observed spatial order of chromosomes in rosettes of mitotic cells.
FAU - Nagele, R G
AU  - Nagele RG
AD  - Department of Molecular Biology, University of Medicine and Dentistry of New 
      Jersey/SOM, Stratford, New Jersey 08084, USA. nagelero@umdnj.edu
FAU - Freeman, T
AU  - Freeman T
FAU - McMorrow, L
AU  - McMorrow L
FAU - Thomson, Z
AU  - Thomson Z
FAU - Kitson-Wind, K
AU  - Kitson-Wind K
FAU - Lee, H y
AU  - Lee Hy
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
SB  - IM
MH  - Cell Cycle/physiology
MH  - Cell Line
MH  - Cell Nucleus/*physiology
MH  - Chromosome Painting
MH  - Chromosomes/*physiology
MH  - Chromosomes, Human, Pair 16/physiology
MH  - Chromosomes, Human, Pair 7/physiology
MH  - Chromosomes, Human, Pair 8/physiology
MH  - Fibroblasts
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
MH  - Mitosis/physiology
MH  - Polyploidy
MH  - Resting Phase, Cell Cycle/*physiology
MH  - X Chromosome/physiology
MH  - Y Chromosome/physiology
EDAT- 1999/01/23 00:00
MHDA- 1999/01/23 00:01
CRDT- 1999/01/23 00:00
PHST- 1999/01/23 00:00 [pubmed]
PHST- 1999/01/23 00:01 [medline]
PHST- 1999/01/23 00:00 [entrez]
AID - 10.1242/jcs.112.4.525 [doi]
PST - ppublish
SO  - J Cell Sci. 1999 Feb;112 ( Pt 4):525-35. doi: 10.1242/jcs.112.4.525.