PMID- 9914294
OWN - NLM
STAT- MEDLINE
DCOM- 19990322
LR  - 20171213
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 81
IP  - 1
DP  - 1999 Jan
TI  - Synaptic organization and neurotransmitters in the rat accessory olfactory bulb.
PG  - 345-55
AB  - The accessory olfactory bulb (AOB) is the first relay station in the vomeronasal 
      system and may play a critical role in processing pheromone signals. The AOB 
      shows similar but less distinct lamination compared with the main olfactory bulb 
      (MOB). In this study, synaptic organization of the AOB was analyzed in slice 
      preparations from adult rats by using both field potential and patch-clamp 
      recordings. Stimulation of the vomeronasal nerve (VN) evoked field potentials 
      that showed characteristic patterns in different layers of the AOB. Current 
      source density (CSD) analysis of the field potentials revealed spatiotemporally 
      separated loci of inward current (sinks) that represented sequential activation 
      of different neuronal components: VN activity (period I), synaptic excitation of 
      mitral cell apical dendrites (period II), and activation of granule cells by 
      mitral cell basal dendrites (period III). Stimulation of the lateral olfactory 
      tract also evoked field potentials in the AOB, which indicated antidromic 
      activation of the mitral cells (period I and II) followed by activation of 
      granule cells (period III). Whole cell patch recordings from mitral and granule 
      cells of the AOB supported that mitral cells are excited by VN terminals and 
      subsequently activate granule cells through dendrodendritic synapses. Both CSD 
      analysis and patch recordings provided evidence that glutamate is the 
      neurotransmitter at the vomeronasal receptor neuron; mitral cell synapses and 
      both NMDA and non-NMDA receptors are involved. We also demonstrated 
      electrophysiologically that reciprocal interaction between mitral and granule 
      cells in the AOB is through the dendrodendritic reciprocal synapses. The 
      neurotransmitter at the mitral-to-granule synapses is glutamate and at the 
      granule-to-mitral synapse is gamma-aminobutyric acid. The synaptic interactions 
      among receptor cell terminals, mitral cells, and granule cells in the AOB are 
      therefore similar to those in the MOB, suggesting that processing of chemosensory 
      information in the AOB shares similarities with that in the MOB.
FAU - Jia, C
AU  - Jia C
AD  - Section of Neurobiology, Yale Medical School, New Haven, Connecticut 06510, USA.
FAU - Chen, W R
AU  - Chen WR
FAU - Shepherd, G M
AU  - Shepherd GM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (Neurotransmitter Agents)
SB  - IM
MH  - Animals
MH  - Electric Stimulation
MH  - Electrophysiology
MH  - Evoked Potentials/physiology
MH  - In Vitro Techniques
MH  - Membrane Potentials/physiology
MH  - Neurons/physiology/ultrastructure
MH  - Neurotransmitter Agents/*physiology
MH  - Olfactory Bulb/cytology/*physiology
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Synapses/*physiology
MH  - Vomeronasal Organ/physiology
EDAT- 1999/01/23 00:00
MHDA- 1999/01/23 00:01
CRDT- 1999/01/23 00:00
PHST- 1999/01/23 00:00 [pubmed]
PHST- 1999/01/23 00:01 [medline]
PHST- 1999/01/23 00:00 [entrez]
AID - 10.1152/jn.1999.81.1.345 [doi]
PST - ppublish
SO  - J Neurophysiol. 1999 Jan;81(1):345-55. doi: 10.1152/jn.1999.81.1.345.