PMID- 9914384
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20190831
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 107
IP  - 6-7
DP  - 1998 Dec
TI  - Aging and diethylstilbestrol-induced aneuploidy in male germ cells: a transgenic 
      mouse model.
PG  - 507-13
AB  - The incidence of aneuploidy in male germ cells was evaluated by analyzing extra 
      marker chromosome(s) signal(s) in round and/or hook spermatids of transgenic 
      mice. Two types of transgenic mice were used as models. The inserted foreign DNA 
      (lambda-gt10LacZ shuttle vector and/or pSVc-myc plasmid) was located at the 
      middle of the long arms of chromosome 2 (lambda DNA) and/or chromosome 8 (c-myc). 
      The number of marker chromosomes present could easily be detected after 
      fluorescence in situ hybridization (FISH) in testicular cells. The frequency of 
      spontaneous aneuploidy of chromosome 2 was similar in round spermatids of lambda 
      and lambda-myc mice. Differential involvement of chromosomes 2 and 8 was observed 
      in both round and hook spermatids. The frequency of spontaneous aneuploidy in 
      round spermatids was higher than that in hook spermatids. The frequency of 
      aneuploidy of marker chromosomes was significantly higher in older mice (2 years 
      old) than in younger ones. Diethylstilbestrol (DES)-induced aneuploidy was dose 
      dependent, and was not influenced by the stage at which germ cells were treated 
      with DES. These results demonstrate the usefulness of a transgenic mouse model 
      for the study of aneuploidy in germ cells.
FAU - Xiao, Y
AU  - Xiao Y
AD  - Department of Radiation Genetics and Chemical Mutagenesis, LUMC, Leiden 
      University, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands.
FAU - Tates, A D
AU  - Tates AD
FAU - Boei JJWA
AU  - Boei JJWA
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 731DCA35BT (Diethylstilbestrol)
SB  - IM
MH  - *Aging
MH  - Aneuploidy
MH  - Animals
MH  - Diethylstilbestrol/*pharmacology
MH  - Germ Cells/*drug effects
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Spermatids/drug effects/ultrastructure
MH  - Spleen/ultrastructure
MH  - Testis/drug effects/ultrastructure
EDAT- 1999/01/23 00:00
MHDA- 1999/01/23 00:01
CRDT- 1999/01/23 00:00
PHST- 1999/01/23 00:00 [pubmed]
PHST- 1999/01/23 00:01 [medline]
PHST- 1999/01/23 00:00 [entrez]
AID - 10.1007/s004120050336 [doi]
PST - ppublish
SO  - Chromosoma. 1998 Dec;107(6-7):507-13. doi: 10.1007/s004120050336.