PMID- 9914386
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20190831
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 107
IP  - 6-7
DP  - 1998 Dec
TI  - Human sperm chromosome complements in chemotherapy patients and infertile men.
PG  - 523-7
AB  - Our studies of human sperm karyotypes and interphase sperm analyzed by 
      fluorescence in situ hybridization (FISH) have both yielded estimates of disomy 
      frequencies of approximately 0.1% per chromosome with an overall aneuploidy 
      frequency in human sperm of approximately 5%-6%. However, the distribution of 
      aneuploidy in sperm is not even, as our data from sperm karyotypes and 
      multicolour FISH analyses both demonstrate a significant increase in the 
      frequency of aneuploidy for chromosome 21 and the sex chromosomes. We have 
      studied men at increased risk of sperm chromosomal abnormalities including cancer 
      patients and infertility patients. Testicular cancer patients were studied before 
      and 2-13 years after chemotherapy (CT) with BEP (bleomycin, etoposide, 
      cisplatin). Sperm karyotype analysis on 788 sperm demonstrated no significant 
      difference in the frequency of numerical or structural chromosomal abnormalities 
      post-CT vs pre-CT. Similarly, multicolour FISH analysis for chromosomes 1, 12, 
      XX, YY and XY in 161,097 sperm did not detect any significant differences in the 
      frequencies of disomy before and after treatment. However, recent evidence has 
      suggested a significant increase in the frequency of disomy and diploidy during 
      CT. We have found that infertile men, who would be candidates for 
      intracytoplasmic sperm injection, have an increased frequency of chromosomally 
      abnormal sperm karyotypes. Also, FISH analysis for chromosomes 1, 12, 13, 21, XX, 
      YY and XY in 255,613 sperm demonstrated a significant increase in chromosomes 1, 
      13, 21, and XY disomy in infertile men compared with control donors.
FAU - Martin, R
AU  - Martin R
AD  - Department of Medical Genetics, University of Calgary, and Department of 
      Genetics, Alberta Children's Hospital, Calgary, Alberta, Canada. 
      Renee@ach.ucalgary.ca
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (Antineoplastic Agents)
RN  - 11056-06-7 (Bleomycin)
RN  - 6PLQ3CP4P3 (Etoposide)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Adult
MH  - Antineoplastic Agents/*adverse effects
MH  - Bleomycin/adverse effects
MH  - *Chromosome Aberrations
MH  - Cisplatin/adverse effects
MH  - Etoposide/adverse effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infertility, Male/*genetics
MH  - Male
MH  - Meiosis
MH  - Middle Aged
MH  - Spermatozoa/ultrastructure
EDAT- 1999/01/23 00:00
MHDA- 1999/01/23 00:01
CRDT- 1999/01/23 00:00
PHST- 1999/01/23 00:00 [pubmed]
PHST- 1999/01/23 00:01 [medline]
PHST- 1999/01/23 00:00 [entrez]
AID - 10.1007/s004120050338 [doi]
PST - ppublish
SO  - Chromosoma. 1998 Dec;107(6-7):523-7. doi: 10.1007/s004120050338.