PMID- 9915579
OWN - NLM
STAT- MEDLINE
DCOM- 19990419
LR  - 20081121
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 214
IP  - 1
DP  - 1999 Jan
TI  - Hepatocyte growth factor expression in the developing myocardium: evidence for a 
      role in the regulation of the mesenchymal cell phenotype and urokinase 
      expression.
PG  - 92-100
AB  - During development of the heart, the endocardium of the developing cushion tissue 
      transforms into mesenchymal cells that rapidly invade the underlying 
      extracellular matrix. This invasive process, along with subsequent proliferation 
      and differentiation of the mesenchyme, are essential for the normal formation of 
      valvular and septal structures. Several factors have been identified that appear 
      to initiate and/or control this transformation process, including the growth 
      factor TGF-beta. In these studies we have investigated whether hepatocyte growth 
      factor/scatter factor (HGF/SF) may also be involved in some aspects of this 
      transformation process. Using an immunohistochemical approach we have detected a 
      spatially restricted localization of HGF/SF to the myocardial cells of the 
      cushion tissue. HGF was detected in extracts of the developing heart, and the 
      presence of the active form correlated with the onset of the transformation 
      process and the elevation of urokinase activity. The endocardial-derived 
      mesenchymal cells of the cushion tissue were found to express the c-met HGF 
      receptor. Isolated endocardial cells responded to the addition of HGF with 
      increases in motility, proliferation, and urokinase production. The results from 
      these studies suggest that HGF may function as a myocardial-derived mediator of 
      the epithelial-mesenchymal transformation by inducing and/or maintaining the 
      mesenchymal cell phenotype. The increase in urokinase expression by the cushion 
      tissue cells, in response to HGF, may facilitate the invasive abilities of these 
      cells and also provide a means of maintaining high levels of active HGF required 
      for the stimulation of cell proliferation and migration.
FAU - Song, W
AU  - Song W
AD  - Department of Cell Biology and Physiology, University of New Mexico School of 
      Medicine, Albuquerque 87131, USA.
FAU - Majka, S M
AU  - Majka SM
FAU - McGuire, P G
AU  - McGuire PG
LA  - eng
GR  - HL46865/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of 
      Anatomists
JID - 9201927
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Animals
MH  - Chick Embryo
MH  - Heart/*embryology
MH  - Hepatocyte Growth Factor/*biosynthesis/genetics
MH  - Mesoderm/*cytology
MH  - Myocardium/*metabolism
MH  - Phenotype
MH  - Quail
MH  - Rabbits
MH  - Urokinase-Type Plasminogen Activator/*metabolism
EDAT- 1999/01/23 19:27
MHDA- 2000/06/22 10:00
CRDT- 1999/01/23 19:27
PHST- 1999/01/23 19:27 [pubmed]
PHST- 2000/06/22 10:00 [medline]
PHST- 1999/01/23 19:27 [entrez]
AID - 10.1002/(SICI)1097-0177(199901)214:1<92::AID-DVDY9>3.0.CO;2-X [pii]
AID - 10.1002/(SICI)1097-0177(199901)214:1<92::AID-DVDY9>3.0.CO;2-X [doi]
PST - ppublish
SO  - Dev Dyn. 1999 Jan;214(1):92-100. doi: 
      10.1002/(SICI)1097-0177(199901)214:1<92::AID-DVDY9>3.0.CO;2-X.