PMID- 9917353
OWN - NLM
STAT- MEDLINE
DCOM- 19990301
LR  - 20141120
IS  - 0011-2240 (Print)
IS  - 0011-2240 (Linking)
VI  - 37
IP  - 4
DP  - 1998 Dec
TI  - Generation of dendritic cells from fresh and frozen cord blood CD34+ cells.
PG  - 362-71
AB  - Dendritic cells (DCs) are professional antigen-presenting cells that are required 
      for the initiation of the immune response. DCs have been shown to be generated 
      from CD34(+) pluripotent hematopoietic progenitor cells in the bone marrow and 
      cord blood (CB), but relatively little is known about the effect of 
      cryopreservation on functional maturation of DCs from hematopoietic stem cells. 
      In this work we report the generation of DCs from cryopreserved CB CD34(+) cells. 
      CB CD34(+) cells were cryopreserved at -80 degreesC for 2 days. Cryopreserved CB 
      CD34(+) cells as well as freshly isolated CB CD34(+) cells cultured with 
      granulocyte-macrophage colony-stimulating factor (GM-CSF)/stem cell factor 
      (SCF)/tumor necrosis factor-alpha (TNF-alpha) for 14 days gave rise to 
      CD1a+/CD4(+)/CD11c+/CD14(-)/CD40(+)/CD80(+ )/CD83(+)/CD86(+)/HLA-DR+ cells with 
      dendritic morphology. DCs derived from cryopreserved CB CD34(+) cells showed a 
      similar endocytic capacity for fluorescein isothiocyanate-labeled dextran and 
      lucifer yellow when compared with DCs derived from freshly isolated CB CD34(+) 
      cells. Flow cytometric analysis revealed that two CC chemokine receptors (CCRs), 
      CCR-1 and CCR-3, were expressed on the cell surface of DCs derived from both 
      cryopreserved and freshly isolated CB CD34(+) cells, and these DCs exhibited 
      similar chemotactic migratory capacities in response to regulated on activation 
      normal T-cell expressed and secreted. DCs derived from cryopreserved as well as 
      freshly isolated CB CD34(+) cells were more efficient than peripheral blood 
      mononuclear cells in the primary allogeneic T-cell response. These results 
      indicate that frozen CB CD34(+) cells cultured with GM-CSF/TNF-alpha/SCF gave 
      rise to dendritic cells which were morphologically, phenotypically and 
      functionally similar to DCs derived from fresh CB CD34(+) cells.
CI  - Copyright 1998 Academic Press.
FAU - Sato, K
AU  - Sato K
AD  - The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, 
      Minato-Ku, Tokyo, 108-8639, Japan.
FAU - Nagayama, H
AU  - Nagayama H
FAU - Takahashi, T A
AU  - Takahashi TA
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Cryobiology
JT  - Cryobiology
JID - 0006252
RN  - 0 (Antigens, CD34)
RN  - 0 (Stem Cell Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Antigens, CD34/*blood
MH  - *Blood Preservation
MH  - Cell Differentiation/drug effects
MH  - Chemotaxis
MH  - *Cryopreservation
MH  - Dendritic Cells/*cytology/drug effects/*immunology
MH  - Endocytosis
MH  - Fetal Blood/*cytology/drug effects/*immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Hematopoietic Stem Cells/cytology/drug effects/immunology
MH  - Humans
MH  - Immunotherapy
MH  - In Vitro Techniques
MH  - Infant, Newborn
MH  - Lymphocyte Activation
MH  - Stem Cell Factor/pharmacology
MH  - T-Lymphocytes/immunology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1999/01/26 00:00
MHDA- 1999/01/26 00:01
CRDT- 1999/01/26 00:00
PHST- 1999/01/26 00:00 [pubmed]
PHST- 1999/01/26 00:01 [medline]
PHST- 1999/01/26 00:00 [entrez]
AID - S0011-2240(98)92136-8 [pii]
AID - 10.1006/cryo.1998.2136 [doi]
PST - ppublish
SO  - Cryobiology. 1998 Dec;37(4):362-71. doi: 10.1006/cryo.1998.2136.