PMID- 9917507
OWN - NLM
STAT- MEDLINE
DCOM- 19990216
LR  - 20190516
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 14
IP  - 2
DP  - 1999 Feb
TI  - Interphase cytogenetic diagnosis of bladder cancer on cells from urine and 
      bladder washing.
PG  - 309-13
AB  - In order to determine the value of fluorescence in situ hybridization (FISH) in 
      the diagnosis and follow-up of bladder cancer interphase cytogenetics was 
      performed on cells from urine and bladder washings. 50 ml of urine or bladder 
      washings were collected. FISH was carried out using centromere probes for 
      chromosomes 7, 8, 9 and 12 according to standard protocols. In each case 100 cell 
      nuclei were analysed. Fifty-four samples from urine and 67 samples from bladder 
      washing were analysed by FISH in comparison with results obtained by conventional 
      cytology. Sensitivity of detection of tumor cells by FISH was 68.5% in urine and 
      63% in bladder washings regardless of tumor stage and grade. Sensitivity obtained 
      by conventional cytology was 50% in urine and 77.3% in bladder washings. FISH on 
      cells from urine samples is an effective complement to the standard urine 
      cytology. Using centromere probes this approach is characterized by high 
      specificity and sensitivity in tumors with T-category higher than pTa and grade 
      higher than G1.
FAU - Junker, K
AU  - Junker K
AD  - Department of Urology, Friedrich-Schiller-University, D-07743 Jena, Germany.
FAU - Werner, W
AU  - Werner W
FAU - Mueller, C
AU  - Mueller C
FAU - Ebert, W
AU  - Ebert W
FAU - Schubert, J
AU  - Schubert J
FAU - Claussen, U
AU  - Claussen U
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
SB  - IM
MH  - Carcinoma, Transitional Cell/*diagnosis/genetics
MH  - Centromere/*genetics
MH  - *Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Urinary Bladder/cytology
MH  - Urinary Bladder Neoplasms/*diagnosis/genetics
MH  - Urine/cytology
EDAT- 1999/01/26 00:00
MHDA- 1999/01/26 00:01
CRDT- 1999/01/26 00:00
PHST- 1999/01/26 00:00 [pubmed]
PHST- 1999/01/26 00:01 [medline]
PHST- 1999/01/26 00:00 [entrez]
AID - 10.3892/ijo.14.2.309 [doi]
PST - ppublish
SO  - Int J Oncol. 1999 Feb;14(2):309-13. doi: 10.3892/ijo.14.2.309.