PMID- 9918455
OWN - NLM
STAT- MEDLINE
DCOM- 19990414
LR  - 20190909
IS  - 0300-9572 (Print)
IS  - 0300-9572 (Linking)
VI  - 39
IP  - 1-2
DP  - 1998 Oct-Nov
TI  - Failure of glutathione peroxidase to reduce transient ischemic injury in the rat 
      hippocampal CA1 subfield.
PG  - 91-8
AB  - It has been postulated that oxygen radical species are produced by 
      ischemia-reperfusion in the brain and play a critical role in neuronal damage. 
      Glutathione peroxidase (GSHPx), one of the antioxidative enzymes, detoxifies 
      hydrogen peroxide, which is the source of a hydroxyl radical that reacts with 
      polyunsaturated fatty acids of the cell membrane, resulting in cell death. The 
      present study was undertaken to investigate the postischemic effect of exogenous 
      GSHPx upon rats subjected to global forebrain ischemia and reperfusion. GSHPx or 
      artificial cerebrospinal fluid (aCSF) as a vehicle for GSHPx was administered 
      into the left cerebral ventricle 15 min after a 5-min 4-vessel occlusion. 
      Neuronal damage and apoptosis were assessed 4 days after ischemic insult using 
      cresyl violet stain and terminal deoxynucleotidyl transferase-mediated dUTP 
      nick-end labeling (TUNEL) method, respectively. Most pyramidal neurons in the 
      hippocampal CA1 subfield were degenerated and their nuclei were stained by the 
      TUNEL in both GSHPx- (80 and 200 units/kg) and aCSF-treated animals. Neurons in 
      other subfields of the hippocampus and dentate gyrus survived. Our further 
      attempt to improve the outcome with a higher dose of GSHPx was unsuccessful 
      because all rats receiving 400 units/kg died soon after the 
      intracerebroventricular injection due to respiratory insufficiency. We conclude 
      that the postischemic treatment with GSHPx does not ameliorate the apoptotic 
      neuronal death of hippocampal CA1 in this transient ischemia model under the 
      conditions used here.
FAU - Yano, T
AU  - Yano T
AD  - Department of Anesthesiology and Surgical Center, Kumamoto University School of 
      Medicine, Honjo, Japan.
FAU - Ushijima, K
AU  - Ushijima K
FAU - Terasaki, H
AU  - Terasaki H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Resuscitation
JT  - Resuscitation
JID - 0332173
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Brain Ischemia/prevention & control
MH  - Glutathione Peroxidase/*pharmacology
MH  - Hippocampus/*blood supply/pathology
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*prevention & control
EDAT- 1999/01/26 00:00
MHDA- 1999/01/26 00:01
CRDT- 1999/01/26 00:00
PHST- 1999/01/26 00:00 [pubmed]
PHST- 1999/01/26 00:01 [medline]
PHST- 1999/01/26 00:00 [entrez]
AID - S0300-9572(98)00115-4 [pii]
AID - 10.1016/s0300-9572(98)00115-4 [doi]
PST - ppublish
SO  - Resuscitation. 1998 Oct-Nov;39(1-2):91-8. doi: 10.1016/s0300-9572(98)00115-4.