PMID- 9918534
OWN - NLM
STAT- MEDLINE
DCOM- 19990211
LR  - 20190623
IS  - 0009-7322 (Print)
IS  - 0009-7322 (Linking)
VI  - 99
IP  - 3
DP  - 1999 Jan 26
TI  - Magnetic resonance imaging and invasive evaluation of development of heart 
      failure in transgenic mice with myocardial expression of tumor necrosis 
      factor-alpha.
PG  - 448-54
AB  - BACKGROUND: Transgenic mice expressing tumor necrosis factor-alpha (TNF-alpha) in 
      cardiac myocytes develop dilated cardiomyopathy, but the temporal progression to 
      cardiac dysfunction is not well characterized. We asked (1) Does magnetic 
      resonance imaging (MRI) provide a reproducible assessment of cardiac output in 
      mice that correlates with invasive measurements obtained with thermodilution? (2) 
      What is the time course of left ventricular (LV) remodeling in transgenic mice 
      with myocardial expression of TNF-alpha? METHODS AND RESULTS: Transgenic mice 
      from 2 different lineages with differing amounts of myocardial TNF-alpha 
      expression [lineage 1 (L1) and lineage 2 (L2)] and littermate controls (LC) were 
      studied. In protocol 1, cardiac output (CO) and stroke volume (SV) were measured 
      by MRI and thermodilution (TD) in 15 mice (3 L1, 4 L2, 8 LC). In protocol 2, 23 
      mice (7 L1, 8 L2, 8 LC) were scanned at 1 month of life and every 4 weeks 
      thereafter. In both protocols, cine-MRI was performed with the use of a 1.5-T 
      clinical system (1.5-mm slices, 195x195 microm in-plane resolution). MRI CO and 
      SV correlated well with TD [COTD (mL/min)=0.94*COMRI+0.72, r=0.84; SVTD( 
      microL)=1. 01*SVMRI-1.07, r=0.94]. Serial MRI studies showed significant increase 
      in LV mass and volumes over time and a significant decrease in ejection fraction 
      in transgenic mice when compared with littermate controls. Compared with lineage 
      2, lineage 1 showed significantly larger LV mass and volumes and significantly 
      lower ejection fraction. CONCLUSIONS: MRI assessment of cardiac function in mice 
      correlates well with invasive measurements. Serial MRI studies in the TNF-alpha 
      mouse model demonstrate that the rate of progression and severity of LV 
      dysfunction are dependent on the degree of TNF-alpha overexpression.
FAU - Franco, F
AU  - Franco F
AD  - Department of Radiology at the University of Texas Southwestern Medical Center at 
      Dallas, 75235-9085, USA.
FAU - Thomas, G D
AU  - Thomas GD
FAU - Giroir, B
AU  - Giroir B
FAU - Bryant, D
AU  - Bryant D
FAU - Bullock, M C
AU  - Bullock MC
FAU - Chwialkowski, M C
AU  - Chwialkowski MC
FAU - Victor, R G
AU  - Victor RG
FAU - Peshock, R M
AU  - Peshock RM
LA  - eng
GR  - 5R29-GM-51822-04/GM/NIGMS NIH HHS/United States
GR  - P01-HL-06296/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Body Weight
MH  - Disease Models, Animal
MH  - Heart Failure/*diagnosis/*genetics/physiopathology
MH  - Heart Rate
MH  - *Magnetic Resonance Imaging
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Myocardium/*chemistry
MH  - Stroke Volume
MH  - Systole/physiology
MH  - Thermodilution
MH  - Tumor Necrosis Factor-alpha/*genetics
MH  - Ventricular Function, Left/physiology
EDAT- 1999/01/26 00:00
MHDA- 1999/01/26 00:01
CRDT- 1999/01/26 00:00
PHST- 1999/01/26 00:00 [pubmed]
PHST- 1999/01/26 00:01 [medline]
PHST- 1999/01/26 00:00 [entrez]
AID - 10.1161/01.cir.99.3.448 [doi]
PST - ppublish
SO  - Circulation. 1999 Jan 26;99(3):448-54. doi: 10.1161/01.cir.99.3.448.