PMID- 9919892
OWN - NLM
STAT- MEDLINE
DCOM- 19990218
LR  - 20190921
IS  - 0933-7407 (Print)
IS  - 0933-7407 (Linking)
VI  - 41
IP  - 11-12
DP  - 1998 Dec
TI  - Comparison of fungal viability assays using Candida albicans yeast cells 
      undergoing prolonged incubation in the absence of nutrients.
PG  - 487-92
AB  - Staining methods for determining fungal viability are usually assessed by 
      comparisons with enumeration of colony-forming units (CFU) on solid media. The 
      purpose of the present study was to compare viability as assessed by the acridine 
      orange (AO) and MTT methods with the numbers of CFUs obtained for Candida 
      albicans yeast cells undergoing prolonged incubation in distilled water. In 
      initial assessments of the assays using various proportions of control and 
      heat-killed C. albicans, the AO and MTT methods consistently indicated 
      significantly higher values for viability than did CFU determinations. 
      Experiments using organisms cultured overnight revealed that approximately 95% of 
      the cells were capable of dividing at least once in a microscopic proliferation 
      assay, whereas only 69% were capable of forming colonies. Parallel assays 
      comparing AO uptake and MTT reduction gave excellent agreement with the 
      microscopic proliferation assay, but not with CFU determinations. Using organisms 
      undergoing prolonged incubations in distilled water, much lower viabilities were 
      obtained with the CFU method at 7 and 10 days than with the microscopic 
      proliferation assay or the two staining methods. These results indicate that the 
      AO and MTT assays correlate well with the ability of C. albicans to divide at 
      least once, but may not accurately indicate the percentage of organisms actually 
      able to form colonies.
FAU - Hjertstedt, J
AU  - Hjertstedt J
AD  - Department of Medicine, Medical College of Wisconsin, Milwaukee, USA.
FAU - Hahn, B L
AU  - Hahn BL
FAU - Kos, W L
AU  - Kos WL
FAU - Sohnle, P G
AU  - Sohnle PG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Mycoses
JT  - Mycoses
JID - 8805008
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 059QF0KO0R (Water)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Candida albicans/*growth & development
MH  - Colony Count, Microbial
MH  - Mycology/*methods
MH  - Tetrazolium Salts
MH  - Thiazoles
MH  - Water
EDAT- 1999/01/27 00:00
MHDA- 1999/01/27 00:01
CRDT- 1999/01/27 00:00
PHST- 1999/01/27 00:00 [pubmed]
PHST- 1999/01/27 00:01 [medline]
PHST- 1999/01/27 00:00 [entrez]
AID - 10.1111/j.1439-0507.1998.tb00711.x [doi]
PST - ppublish
SO  - Mycoses. 1998 Dec;41(11-12):487-92. doi: 10.1111/j.1439-0507.1998.tb00711.x.