PMID- 9925207
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190522
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 11
IP  - 1
DP  - 1999 Jan
TI  - Seroneutralization of porcine reproductive and respiratory syndrome virus 
      correlates with antibody response to the GP5 major envelope glycoprotein.
PG  - 20-6
AB  - To determine the structural protein of the porcine reproductive and respiratory 
      syndrome virus (PRRSV) involved in the production of neutralizing antibodies 
      following clinical infection, correlation was studied between virus 
      neutralization capability of convalescent pig sera and antibody response to the 
      open reading frames (ORFs) 3-, 4-, 5-, and 7-encoded proteins GP3, GP4, GP5, and 
      N, respectively. Individual virus genes were cloned into the pGEX-4T-1 vector, 
      and the recombinant viral proteins were expressed in Escherichia coli fused to 
      the glutathione S-transferase (GST) protein. The resulting GST-ORF3, GST-ORF4, 
      GST-ORF5, and GST-ORF7 recombinant fusion proteins were purified by 
      electroelution and used as antigens for serologic testing by indirect 
      enzyme-linked immunosorbent assay and western immunoblotting. The overall 
      antibody (IgG and IgM) titers to PRRSV of pooled convalescent pig sera were first 
      determined by indirect immunofluorescence, and then sera with specific IgG titers 
      > 1:1,024 were tested for their specific virus neutralization activity and 
      reactivity to individual recombinant fusion proteins. Except for the early immune 
      response (as revealed by the presence of specific IgM), neutralizing titers were 
      correlated with anti-GP5 titers but not with anti-GP3 and anti-GP4 titers. The 
      correlation between virus neutralization and anti-GP5 titers was significant (r = 
      0.811, P < or = 0.001).
FAU - Gonin, P
AU  - Gonin P
AD  - Center for Research in Virology, Institut Armand-Frappier, Universite du Quebec, 
      Laval-des-Rapides, Canada.
FAU - Pirzadeh, B
AU  - Pirzadeh B
FAU - Gagnon, C A
AU  - Gagnon CA
FAU - Dea, S
AU  - Dea S
LA  - eng
SI  - GENBANK/AF003343
SI  - GENBANK/AF003345
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA Primers)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Antibody Formation
MH  - DNA Primers
MH  - Immunoglobulin G/*blood
MH  - Immunoglobulin M/*blood
MH  - Neutralization Tests
MH  - Open Reading Frames
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Recombinant Fusion Proteins/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine
MH  - Viral Envelope Proteins/genetics/*immunology
EDAT- 1999/01/30 00:00
MHDA- 1999/01/30 00:01
CRDT- 1999/01/30 00:00
PHST- 1999/01/30 00:00 [pubmed]
PHST- 1999/01/30 00:01 [medline]
PHST- 1999/01/30 00:00 [entrez]
AID - 10.1177/104063879901100103 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 1999 Jan;11(1):20-6. doi: 10.1177/104063879901100103.