PMID- 9925929
OWN - NLM
STAT- MEDLINE
DCOM- 19990305
LR  - 20171101
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 83
IP  - 1-2
DP  - 1998
TI  - Differential destabilization of repetitive sequence hybrids in fluorescence in 
      situ hybridization.
PG  - 60-3
AB  - A method for painting a chromosome or chromosome region by fluorescence in situ 
      hybridization (FISH) without blocking DNA is described. Both unique sequence and 
      repetitive sequence components of a fluorescently labeled probe are hybridized 
      under low-stringency conditions, but the chromosomes are washed in such a manner 
      that repetitive sequences are differentially removed, while region-specific 
      unique sequence fragments remain bound to the target chromosomes. We refer to 
      this differential retention and removal of probe components as differential 
      stability FISH.
FAU - Hozier, J C
AU  - Hozier JC
AD  - Applied Genetics Laboratories, Inc., Melbourne, FL, (USA). jh@appliedgenetics.com
FAU - Scalzi, J M
AU  - Scalzi JM
FAU - Clase, A C
AU  - Clase AC
FAU - Davis, L M
AU  - Davis LM
FAU - Liechty, M C
AU  - Liechty MC
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Salts)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/genetics
MH  - Chromosome Mapping/*methods
MH  - DNA Probes
MH  - DNA, Neoplasm/analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Hybridization/methods
MH  - Receptor, ErbB-2/genetics
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Salts
MH  - Temperature
EDAT- 1999/02/02 03:01
MHDA- 2000/08/16 11:00
CRDT- 1999/02/02 03:01
PHST- 1999/02/02 03:01 [pubmed]
PHST- 2000/08/16 11:00 [medline]
PHST- 1999/02/02 03:01 [entrez]
AID - 15127 [pii]
AID - 10.1159/000015127 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1998;83(1-2):60-3. doi: 10.1159/000015127.