PMID- 9927485
OWN - NLM
STAT- MEDLINE
DCOM- 19990305
LR  - 20181113
IS  - 1088-9051 (Print)
IS  - 1088-9051 (Linking)
VI  - 9
IP  - 1
DP  - 1999 Jan
TI  - Mapping ESTs by fiber-FISH.
PG  - 62-71
AB  - A visual transcript map of six genes was constructed on the chromosome 21q22.3 by 
      high resolution fluorescence in situ hybridization (FISH). Expressed sequence 
      tags (ESTs) from six genes-PWP2, KNP1, AIRE, C21orf3, SMT3A, and C21orf1-were 
      successfully localized by fiber-FISH by use of sensitive tyramide-based 
      detection. The sizes of the ESTs varied between 315 to 956 bp and most of them 
      map within the 3'-untranslated region. The ESTs were assigned to and subsequently 
      ordered within cosmid, PAC, and BAC clones hybridized on DNA fibers. Physical 
      distances between ESTs and known markers were determined. Our results demonstrate 
      the feasibility and accuracy of visual mapping EST sequences in relation to known 
      markers. The main advantage of this approach is that it can be applied to finely 
      map any of the database ESTs for positional cloning efforts. The sensitivity, 
      specificity, and reproducibility of this high-resolution EST mapping technique is 
      evaluated.
FAU - Horelli-Kuitunen, N
AU  - Horelli-Kuitunen N
AD  - Department of Clinical Chemistry, University of Helsinki and Laboratory, 
      Department of Helsinki University Central Hospital, 00290 Helsinki, Finland. 
      Nina.Horelli-Kuitunen@HUCH.fi
FAU - Aaltonen, J
AU  - Aaltonen J
FAU - Yaspo, M L
AU  - Yaspo ML
FAU - Eeva, M
AU  - Eeva M
FAU - Wessman, M
AU  - Wessman M
FAU - Peltonen, L
AU  - Peltonen L
FAU - Palotie, A
AU  - Palotie A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genome Res
JT  - Genome research
JID - 9518021
RN  - 0 (3' Untranslated Regions)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Human, Pair 21/genetics
MH  - Contig Mapping
MH  - *Expressed Sequence Tags
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Transcription, Genetic
PMC - PMC310701
EDAT- 1999/02/02 00:00
MHDA- 1999/02/02 00:01
PMCR- 1999/07/01
CRDT- 1999/02/02 00:00
PHST- 1999/02/02 00:00 [pubmed]
PHST- 1999/02/02 00:01 [medline]
PHST- 1999/02/02 00:00 [entrez]
PHST- 1999/07/01 00:00 [pmc-release]
PST - ppublish
SO  - Genome Res. 1999 Jan;9(1):62-71.