PMID- 9927689
OWN - NLM
STAT- MEDLINE
DCOM- 19990305
LR  - 20230331
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 3
DP  - 1999 Feb 2
TI  - Distinct dendritic cell subsets differentially regulate the class of immune 
      response in vivo.
PG  - 1036-41
AB  - Dendritic cells (DCs) are unique in their ability to stimulate T cells and 
      initiate adaptive immunity. Injection of mice with the cytokine Flt3-ligand (FL) 
      dramatically expands mature lymphoid and myeloid-related DC subsets. In contrast, 
      injection of a polyethylene glycol-modified form of granulocyte/macrophage 
      colony-stimulating factor (GM-CSF) into mice only expands the myeloid-related DC 
      subset. These DC subsets differ in the cytokine profiles they induce in T cells 
      in vivo. The lymphoid-related subset induces high levels of the Th1 cytokines 
      interferon gamma and interleukin (IL)-2 but little or no Th2 cytokines. In 
      contrast, the myeloid-related subset induces large amounts of the Th2 cytokines 
      IL-4 and IL-10, in addition to interferon gamma and IL-2. FL- or GM-CSF-treated 
      mice injected with soluble ovalbumin display dramatic increases in 
      antigen-specific antibody titers, but the isotype profiles seem critically 
      dependent on the cytokine used. Although FL treatment induces up to a 10, 
      000-fold increase in ovalbumin-specific IgG2a and a more modest increase in IgG1 
      titers, GM-CSF treatment favors a predominantly IgG1 response with little 
      increase in IgG2a levels. These data suggest that distinct DC subsets have 
      strikingly different influences on the type of immune response generated in vivo 
      and may thus be targets for pharmacological intervention.
FAU - Pulendran, B
AU  - Pulendran B
AD  - Immunex Corporation, 51 University Street, Seattle, WA 98101, USA.
FAU - Smith, J L
AU  - Smith JL
FAU - Caspary, G
AU  - Caspary G
FAU - Brasel, K
AU  - Brasel K
FAU - Pettit, D
AU  - Pettit D
FAU - Maraskovsky, E
AU  - Maraskovsky E
FAU - Maliszewski, C R
AU  - Maliszewski CR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Membrane Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (flt3 ligand protein)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Bone Marrow Cells/cytology/immunology
MH  - CHO Cells
MH  - Cricetinae
MH  - Crosses, Genetic
MH  - Dendritic Cells/classification/drug effects/*immunology
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Lymphoid Tissue/cytology/immunology
MH  - Male
MH  - Membrane Proteins/*immunology/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, SCID
MH  - Recombinant Proteins/immunology/pharmacology
MH  - Species Specificity
MH  - T-Lymphocytes/immunology
PMC - PMC15346
EDAT- 1999/02/03 00:00
MHDA- 1999/02/03 00:01
PMCR- 1999/08/02
CRDT- 1999/02/03 00:00
PHST- 1999/02/03 00:00 [pubmed]
PHST- 1999/02/03 00:01 [medline]
PHST- 1999/02/03 00:00 [entrez]
PHST- 1999/08/02 00:00 [pmc-release]
AID - 4617 [pii]
AID - 10.1073/pnas.96.3.1036 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Feb 2;96(3):1036-41. doi: 10.1073/pnas.96.3.1036.