PMID- 9929513
OWN - NLM
STAT- MEDLINE
DCOM- 19990407
LR  - 20100908
IS  - 0090-9556 (Print)
IS  - 0090-9556 (Linking)
VI  - 27
IP  - 2
DP  - 1999 Feb
TI  - Developmental expression of N-acetyltransferases in C57BI/6 mice.
PG  - 261-4
AB  - N-Acetyltransferases (NATs) play an important role in the biotransformation of a 
      wide variety of arylamine drugs and carcinogens. Two genes (NAT1, NAT2) have been 
      identified and allelic variation in NAT2 has been associated with arylamine 
      toxicity in adults. Little information has been reported on expression of NAT 
      genes during embryonic and fetal development although substrate specific NAT 
      activity has been detected. The current study investigated the expression of NAT1 
      and NAT2 in mice pre-and postnatally. RNA was isolated from maternal liver, 
      embryonic tissue at gestational days (GD) 10, 15, and 18, or neonates at neonatal 
      day 3. Reverse transcription-polymerase chain reaction was performed using 
      primers designed to amplify portions of either the NAT1 or the NAT2 gene. NAT1 
      and NAT2 mRNAs were detected in the embryo/placental complex at GD 10 and in GD 
      15 and 18 embryos. NAT2 but not NAT1 was expressed in GD 18 and neonatal day 3 
      hepatic tissue. These data demonstrate the differential expression of NAT genes 
      in the mouse embryo and suggest a potential role for NAT in development.
FAU - Mitchell, M K
AU  - Mitchell MK
AD  - Department of Pharmacology and Toxicology, The University of Arizona, Tucson 
      85721, USA.
FAU - Futscher, B W
AU  - Futscher BW
FAU - McQueen, C A
AU  - McQueen CA
LA  - eng
GR  - P30ES06694/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Drug Metab Dispos
JT  - Drug metabolism and disposition: the biological fate of chemicals
JID - 9421550
RN  - 0 (Isoenzymes)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.5 (Arylamine N-Acetyltransferase)
RN  - EC 2.3.1.5 (N-acetyltransferase 1)
RN  - EC 2.3.1.5 (NAT2 protein, human)
SB  - IM
MH  - Acetyltransferases/*biosynthesis/genetics
MH  - Animals
MH  - Animals, Newborn
MH  - Arylamine N-Acetyltransferase/biosynthesis/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Embryo, Mammalian/metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Gene Expression Regulation, Enzymologic
MH  - Isoenzymes
MH  - Liver/embryology/enzymology/growth & development
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pregnancy
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/02/04 00:00
MHDA- 1999/02/04 00:01
CRDT- 1999/02/04 00:00
PHST- 1999/02/04 00:00 [pubmed]
PHST- 1999/02/04 00:01 [medline]
PHST- 1999/02/04 00:00 [entrez]
PST - ppublish
SO  - Drug Metab Dispos. 1999 Feb;27(2):261-4.