PMID- 9930487
OWN - NLM
STAT- MEDLINE
DCOM- 19990218
LR  - 20190628
IS  - 0003-4975 (Print)
IS  - 0003-4975 (Linking)
VI  - 66
IP  - 6
DP  - 1998 Dec
TI  - Immunogenic human leukocyte antigen class II antigens on human cardiac valves 
      induce specific alloantibodies.
PG  - 2022-6
AB  - BACKGROUND: The kinetics of panel reactive antibodies (PRA) and incidence of 
      antibodies directed against human leukocyte antigen (HLA) class II were studied 
      in patients who received a cryopreserved cardiac valve allograft. METHODS: A 
      complement-dependent microlymphocytotoxicity test was used to determine the 
      percentage of panel reactive antibodies. Anti-HLA class II antibodies were 
      measured by two-color fluorescence assays. RESULTS: The panel reactive antibodies 
      became positive in 25 (78%) of 32 recipients between 1 and 16 months after 
      implantation. Antibodies against HLA class II antigens were detected in 11 (37%) 
      of 30 patients. In 9 (82%) of 11 cases these antibodies were donor specific. The 
      induction of antibodies against donor HLA class II antigens suggests that intact 
      HLA class II antigens are expressed by viable cells within the graft. 
      Dithiothreitol analysis showed that the antibodies were of the immunoglobulin G 
      type. Apparently, the HLA class II antigens are expressed in an immunogenic way, 
      as activation of specific T-helper cells is essential for the switch from 
      immunoglobulin M to immunoglobulin G antibodies. CONCLUSIONS: Allogeneic valve 
      transplantation is associated with the production of donor-specific anti-HLA 
      class I and II antibodies that could contribute to graft failure. This possibly 
      detrimental effect might be prevented by cross matching in sensitized patients.
FAU - Hoekstra, F M
AU  - Hoekstra FM
AD  - Department of Internal Medicine I, University Hospital Rotterdam, The 
      Netherlands. hoekstra@inw1a2r.nl
FAU - Witvliet, M
AU  - Witvliet M
FAU - Knoop, C Y
AU  - Knoop CY
FAU - Wassenaar, C
AU  - Wassenaar C
FAU - Bogers, A J
AU  - Bogers AJ
FAU - Weimar, W
AU  - Weimar W
FAU - Claas, F H
AU  - Claas FH
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Ann Thorac Surg
JT  - The Annals of thoracic surgery
JID - 15030100R
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Adult
MH  - *Antibody Specificity
MH  - Aortic Valve/immunology/*transplantation
MH  - Cryopreservation
MH  - Female
MH  - Histocompatibility Antigens Class I/immunology
MH  - Histocompatibility Antigens Class II/*immunology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Isoantibodies/*immunology
MH  - Male
MH  - Pulmonary Valve/immunology/*transplantation
MH  - Transplantation, Homologous
EDAT- 1999/02/04 00:00
MHDA- 1999/02/04 00:01
CRDT- 1999/02/04 00:00
PHST- 1999/02/04 00:00 [pubmed]
PHST- 1999/02/04 00:01 [medline]
PHST- 1999/02/04 00:00 [entrez]
AID - S0003-4975(98)01058-3 [pii]
AID - 10.1016/s0003-4975(98)01058-3 [doi]
PST - ppublish
SO  - Ann Thorac Surg. 1998 Dec;66(6):2022-6. doi: 10.1016/s0003-4975(98)01058-3.