PMID- 9930921
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20191102
IS  - 0946-2716 (Print)
IS  - 0946-2716 (Linking)
VI  - 77
IP  - 1
DP  - 1999 Jan
TI  - Monoclonal antibody to tumor necrosis factor-alpha modulates hepatocellular Ca2+ 
      homeostasis during hemorrhagic shock in the rat.
PG  - 8-13
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a key mediator of shock-induced 
      cellular and humoral inflammatory cascades. The present study investigated the 
      role of TNF-alpha in oxidative membrane injury and altered hepatocyte Ca2+ 
      regulation, both of which are critical steps in cellular dysfunction during 
      ischemia/reperfusion events. Hemorrhagic shock was induced by bleeding male 
      Sprague-Dawley rats (200-250 g, n=6/group) to a mean arterial blood pressure of 
      40 mmHg for 60 min. Rats were resuscitated with 60% of shed blood and twice the 
      shed blood volume as Ringers' lactate. At the end of hemorrhage and 60 min after 
      resuscitation, hepatocytes were isolated by liver collagenase perfusion. 
      Hepatocyte Ca2+ uptake (Ca2+up) and Ca2+ membrane flux (Ca2+flux) were determined 
      by 45Ca2+ incubation techniques. Hepatocyte reduced/oxidized glutathione and 
      lipid peroxidation were determined fluorometrically. Both hemorrhage and 
      hemorrhage/resuscitation significantly increased hepatocyte Ca2+up and Ca2+flux. 
      The monoclonal chimeric mouse gamma1 TNF-alpha antibody (TN3gamma1.19.12; 20 
      mg/kg b.w.) given with resuscitation significantly decreased hepatocyte Ca2+up 
      and Ca2+flux and prevented hepatocyte lipid peroxidation. These findings suggest 
      that oxidative membrane injury could be the result of TNF-alpha modulation of 
      hepatocellular Ca2+ regulation during hemorrhage/resuscitation.
FAU - Pizanis, A
AU  - Pizanis A
AD  - Department of Trauma, Hand and Reconstructive Surgery, University of Saarland, 
      Homburg/Saar, Germany.
FAU - Mutschler, W
AU  - Mutschler W
FAU - Rose, S
AU  - Rose S
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - J Mol Med (Berl)
JT  - Journal of molecular medicine (Berlin, Germany)
JID - 9504370
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - GAN16C9B8O (Glutathione)
RN  - SY7Q814VUP (Calcium)
SB  - IM
CIN - J Mol Med (Berl). 1999 Jan;77(1):1-2. PMID: 9930919
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Calcium/*metabolism
MH  - Glutathione/metabolism
MH  - Homeostasis
MH  - Lipid Peroxidation
MH  - Liver/cytology/*metabolism
MH  - Male
MH  - Oxidation-Reduction
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Shock, Hemorrhagic/metabolism/*physiopathology
MH  - Tumor Necrosis Factor-alpha/immunology/*physiology
EDAT- 1999/02/04 00:00
MHDA- 1999/02/04 00:01
CRDT- 1999/02/04 00:00
PHST- 1999/02/04 00:00 [pubmed]
PHST- 1999/02/04 00:01 [medline]
PHST- 1999/02/04 00:00 [entrez]
AID - 10.1007/s001090050291 [doi]
PST - ppublish
SO  - J Mol Med (Berl). 1999 Jan;77(1):8-13. doi: 10.1007/s001090050291.