PMID- 9931487
OWN - NLM
STAT- MEDLINE
DCOM- 19990312
LR  - 20220227
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 64
IP  - 2
DP  - 1999 Feb 5
TI  - Identification and cloning of three novel human G protein-coupled receptor genes 
      GPR52, PsiGPR53 and GPR55: GPR55 is extensively expressed in human brain.
PG  - 193-8
AB  - The G protein-coupled receptor (GPCR) family share a structural motif of seven 
      transmembrane segments with large numbers of conserved residues in those regions. 
      Here, we report the identification and cloning of two novel human intronless GPCR 
      genes, GPR52, GPR55 and a pseudogene PsiGPR53. GPR55 was identified from the 
      expressed sequence tags (EST) database whereas GPR52 and pseudogene PsiGPR53 
      originated from the high throughput genome (HTG) database. A partial cDNA clone 
      obtained from the IMAGE Consortium of GPR55 was used to screen a human genomic 
      library to acquire the full length gene. GPR52 and PsiGPR53 were amplified from 
      human genomic DNA using primers based on the HTG sequences. GPR55 and GPR52 
      encode receptors of 319 and 361 amino acids, respectively. GPR55 gene was mapped 
      to chromosome 2q37, using fluorescence in situ hybridization (FISH), and its mRNA 
      transcripts have been detected in the caudate nucleus and putamen, but not in 
      five other brain regions. Human receptors showing the highest amino acid identity 
      to GPR55 include P2Y5 (29%), GPR23 (30%), GPR35 (27%) and CCR4 (23%). GPR52 gene 
      localized to chromosome 1q24 shares the highest identity with GPR21 (71%), 
      histamine H2 (27%) and 5-HT4 (26%) human receptors. PsiGPR53 is a pseudogene 
      mapped to chromosome 6p21 that demonstrates the highest similarity to the MRG 
      (35%), MAS (28%) and C5a (24%) human receptor genes.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Sawzdargo, M
AU  - Sawzdargo M
AD  - Department of Pharmacology, University of Toronto, Medical Sciences Building, 
      Toronto, Ontario, Canada, USA.
FAU - Nguyen, T
AU  - Nguyen T
FAU - Lee, D K
AU  - Lee DK
FAU - Lynch, K R
AU  - Lynch KR
FAU - Cheng, R
AU  - Cheng R
FAU - Heng, H H
AU  - Heng HH
FAU - George, S R
AU  - George SR
FAU - O'Dowd, B F
AU  - O'Dowd BF
LA  - eng
SI  - GENBANK/AF096784
SI  - GENBANK/AF096785
SI  - GENBANK/AF096786
SI  - GENBANK/AF100789
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Receptors, Cell Surface)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Brain/*metabolism
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Databases, Factual
MH  - GTP-Binding Proteins/*metabolism
MH  - Gene Expression Regulation/*physiology
MH  - Genome, Human
MH  - Humans
MH  - Molecular Sequence Data
MH  - Receptors, Cell Surface/*genetics
EDAT- 1999/02/05 00:00
MHDA- 1999/02/05 00:01
CRDT- 1999/02/05 00:00
PHST- 1999/02/05 00:00 [pubmed]
PHST- 1999/02/05 00:01 [medline]
PHST- 1999/02/05 00:00 [entrez]
AID - S0169328X98002770 [pii]
AID - 10.1016/s0169-328x(98)00277-0 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Feb 5;64(2):193-8. doi: 
      10.1016/s0169-328x(98)00277-0.