PMID- 9933437
OWN - NLM
STAT- MEDLINE
DCOM- 19990218
LR  - 20220311
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 115
IP  - 1
DP  - 1999 Jan
TI  - CD40 engagement modulates the production of matrix metalloproteinases by gingival 
      fibroblasts.
PG  - 161-7
AB  - Chronic periodontitis is a destructive inflammatory disease linked with 
      unbalanced production between matrix metalloproteinases (MMPs), such as 
      interstitial collagenase (MMP-1) and stromelysin-1 (MMP-3) and their endogenous 
      tissue inhibitors of MMPs (TIMPs). In addition to aberrant MMP-1 and MMP-3 
      expression, periodontal lesions are characterized by dense infiltrations of 
      activated T lymphocytes which may interact with CD40-expressing gingival 
      fibroblasts in the connective tissue via the CD40L-CD40 pathway. In this study we 
      investigated whether CD40 cross-linking influenced MMP production by gingival 
      fibroblasts. Therefore, we analysed the CD40L-induced MMP production by these 
      fibroblasts in the presence of cytokines that are increased in periodontal 
      lesions, such as IL-1beta, tumour necrosis factor-alpha (TNF-alpha) and 
      interferon-gamma (IFN-gamma). We show that CD40 ligation on gingival fibroblasts 
      resulted in a decrease of their MMP-1 and MMP-3 production, while MMP-2 and 
      TIMP-1 production were unaffected as determined by Western blot. This 
      down-regulatory effect of CD40 engagement on MMP-1 and MMP-3 production by 
      gingival fibroblasts was also present when MMP production was up-regulated by 
      IL-1beta and TNF-alpha or down-regulated by IFN-gamma. These results suggest that 
      CD40 ligation on gingival fibroblasts leads to a restraining of MMP-1 and MMP-3 
      production by gingival fibroblasts and thereby may be an important mechanism in 
      the retardation of further periodontal tissue damage.
FAU - Wassenaar, A
AU  - Wassenaar A
AD  - Department of Cell Biology and Histology, Academic Medical Centre (AMC), 
      University of Amsterdam, The Netherlands.
FAU - Verschoor, T
AU  - Verschoor T
FAU - Kievits, F
AU  - Kievits F
FAU - Den Hartog, M T
AU  - Den Hartog MT
FAU - Kapsenberg, M L
AU  - Kapsenberg ML
FAU - Everts, V
AU  - Everts V
FAU - Snijders, A
AU  - Snijders A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (CD40 Antigens)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.4.24.- (Metalloendopeptidases)
SB  - IM
MH  - Adult
MH  - CD40 Antigens/*physiology
MH  - Extracellular Matrix/*enzymology
MH  - Fibroblasts/metabolism/*physiology
MH  - Gingiva/*cytology
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-1/pharmacology
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Metalloendopeptidases/*biosynthesis
MH  - Tumor Necrosis Factor-alpha/pharmacology
PMC - PMC1905194
EDAT- 1999/02/05 00:00
MHDA- 1999/02/05 00:01
PMCR- 2000/01/01
CRDT- 1999/02/05 00:00
PHST- 1999/02/05 00:00 [pubmed]
PHST- 1999/02/05 00:01 [medline]
PHST- 1999/02/05 00:00 [entrez]
PHST- 2000/01/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2249.1999.00764.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1999 Jan;115(1):161-7. doi: 10.1046/j.1365-2249.1999.00764.x.