PMID- 9933824
OWN - NLM
STAT- MEDLINE
DCOM- 19990323
LR  - 20171101
IS  - 1420-4096 (Print)
IS  - 1420-4096 (Linking)
VI  - 21
IP  - 6
DP  - 1998
TI  - Calcium-sensing receptor: regulation of electrolyte transport in the thick 
      ascending limb of Henle's loop.
PG  - 401-12
AB  - A calcium-sensing receptor (CaR) has functionally been described in the cortical 
      thick ascending limb of Henle's loop (CTAL) of rat and mouse. This G 
      protein-coupled receptor activates phospholipase C and increases the 
      intracellular Ca2+ concentration. We observed that in the mouse CTAL cAMP 
      formation, induced by 10(-8) mol/l AVP, was inhibited by more than 90% when the 
      extracellular Ca2+ concentration ([Ca2+]e) was increased from 0.5 to 3 mmol/l. 
      Measurements of transepithelial potential difference (PDte) in rat and mouse CTAL 
      and medullary thick ascending limb (mTAL) segments and of transepithelial ion net 
      fluxes in the mouse CTAL (isotonic perfusion conditions: 150 mmol/l NaCl in the 
      lumen and bath) showed that an increase in the [Ca2+]e had no effect on basal and 
      arginine vasopressin (AVP, 10(-10) mol/l)-stimulated transepithelial PDte, NaCl 
      and Mg2+ transport. However, Ca2+ reabsorption was strongly inhibited by 
      increased [Ca2+]e. Addition of AVP reversed this inhibitory effect of increased 
      [Ca2+]e. Under hypotonic perfusion conditions (lumen 50 mmol/l NaCl; bath 150 
      mmol/l NaCl), a high [Ca2+]e induced a 50% decrease in Mg2+ reabsorption which 
      was restored by AVP. Under these conditions, the effects on Ca2+ transport 
      described above were still observed. In conclusion, activation of the CaR in the 
      mouse TAL has no effect on basal and AVP-stimulated transepithelial NaCl 
      reabsorption despite its large inhibitory effect on cAMP synthesis. The CaR, 
      however, could play a role in the regulation of transepithelial Ca2+ and Mg2+ 
      reabsorption.
FAU - Desfleurs, E
AU  - Desfleurs E
AD  - URA CNRS 1859, Departement de Biologie Cellulaire et Moleculaire, CEA Saclay, 
      Gif-sur-Yvette, France.
FAU - Wittner, M
AU  - Wittner M
FAU - Simeone, S
AU  - Simeone S
FAU - Pajaud, S
AU  - Pajaud S
FAU - Moine, G
AU  - Moine G
FAU - Rajerison, R
AU  - Rajerison R
FAU - Di Stefano, A
AU  - Di Stefano A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Kidney Blood Press Res
JT  - Kidney & blood pressure research
JID - 9610505
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Electrolytes)
RN  - 113-79-1 (Arginine Vasopressin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - Absorption
MH  - Animals
MH  - Arginine Vasopressin/pharmacology
MH  - Biological Transport
MH  - Calcium-Binding Proteins/*physiology
MH  - Cyclic AMP/metabolism
MH  - Electrolytes/*pharmacokinetics
MH  - Female
MH  - Hypercalcemia/metabolism
MH  - Loop of Henle/*metabolism
MH  - Magnesium/pharmacokinetics
MH  - Mice
MH  - Rats
MH  - Stimulation, Chemical
EDAT- 1999/02/06 03:13
MHDA- 2000/08/16 11:00
CRDT- 1999/02/06 03:13
PHST- 1999/02/06 03:13 [pubmed]
PHST- 2000/08/16 11:00 [medline]
PHST- 1999/02/06 03:13 [entrez]
AID - 25892 [pii]
AID - 10.1159/000025892 [doi]
PST - ppublish
SO  - Kidney Blood Press Res. 1998;21(6):401-12. doi: 10.1159/000025892.