PMID- 993590
OWN - NLM
STAT- MEDLINE
DCOM- 19770129
LR  - 20061115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 117
IP  - 5 Pt.2
DP  - 1976 Nov
TI  - Preparation and subfractionation of isologous and heterologous anti-idiotypes, 
      using FV fragments.
PG  - 1933-7
AB  - Fv fragments prepared from the BALB/c anti-dinitrophenyl (DNP) myeloma proteins, 
      produced by plasmacytoma MOPC 315 and XRPC 25, were used to raise anti-idiotypic 
      antibodies in rabbits. Anti-idiotypes to these proteins were also prepared in the 
      syngeneic BALB/c mice by injecting the intact proteins, whereas Fv fragment 
      failed to induce antibodies in these mice. Rabbit anti-Fv of late bleedings did 
      not show the presence of antibodies to "framework determinants" (determinants 
      common to all V regions of mouse immunoglobulins), and they contained 
      precipitating anti-idiotypes whereas mouse antisera contained non-precipitating 
      anti-idiotypes. No cross-reaction was observed between anti-idiotypes to these 
      myeloma proteins and induced anti-DNP antibodies from BALB/c mice, when either 
      rabbit or mouse antisera were analyzed. The anti-sera were fractionated into 
      anti-binding site and anti-non-binding site by adsorption onto the 
      immunogen-Sepharose column and elution by DNP lysine, followed by elution with 
      acetic acid. Only the anti-binding sites were inhibited by hapten in their 
      interaction with the homologous myeloma protein. The rabbit anti-Fv antibodies 
      were predominantly (over 90%) anti-non-binding site whereas the mouse 
      anti-idiotypes were predominantly (60 to 70%) anti-binding site.
FAU - Helman, M
AU  - Helman M
FAU - Shreier, I
AU  - Shreier I
FAU - Givol, D
AU  - Givol D
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies)
RN  - 0 (Immune Sera)
RN  - 0 (Immunoglobulin Fragments)
SB  - IM
MH  - Animals
MH  - Antibodies/analysis
MH  - Chemical Fractionation
MH  - Cross Reactions
MH  - Immune Sera/*isolation & purification
MH  - *Immunoglobulin Fragments
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Rabbits
MH  - Species Specificity
EDAT- 1976/11/01 00:00
MHDA- 1976/11/01 00:01
CRDT- 1976/11/01 00:00
PHST- 1976/11/01 00:00 [pubmed]
PHST- 1976/11/01 00:01 [medline]
PHST- 1976/11/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1976 Nov;117(5 Pt.2):1933-7.