PMID- 9949239
OWN - NLM
STAT- MEDLINE
DCOM- 19990504
LR  - 20061115
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 16
IP  - 1
DP  - 1999 Feb 10
TI  - [A DNA probe suitable for the detection of chromosome 21 copy number in human 
      interphase nuclei by fluorescence in situ hybridization].
PG  - 36-40
AB  - OBJECTIVE: To prepare DNA probe which can be used to accurately detect chromosome 
      21 copy number in human interphase nuclei by fluorescence in situ hybridization 
      (FISH). METHODS: A probe was produced by universal primer PCR from YAC clone 
      881D2 and characterized by FISH to metaphases and interphase nuclei of peripheral 
      lymphocytes from 8 normal individuals and 5 patients suffering from trisomy 21. 
      RESULTS: A DNA probe was produced and its characteristics were :(1) Most of the 
      probes ranged in size from 350bp to 750bp; (2) The hybridization signals of the 
      probes were located near centromere on long arm of human chromosome 21; (3) In 
      1524 metaphases scored, about 99.95% of hybridization signals were located on 
      chromosome 21; (4) The signals were very bright and recognizable easily in both 
      metaphases and interphases nuclei; (5) The expected copy number of chromosome 21 
      was detected by FISH with the probe in more than 98.40% of interphase nuclei and 
      99.60% of metaphases. CONCLUSION: The DNA probe reported here is suitable for the 
      detection of chromosome 21 copy number in interphase nuclei and for the study of 
      segregation of chromosome 21 during mitoses of human lymphocytes in culture.
FAU - Shi, Q
AU  - Shi Q
AD  - Department of Biology, Nanjing Normal University, Nanjing, Jiangsu, 210097 P. R. 
      China.
FAU - Shan, X
AU  - Shan X
FAU - Zhang, J
AU  - Zhang J
FAU - Zhang, X
AU  - Zhang X
FAU - Chen, Y
AU  - Chen Y
FAU - Deng, X
AU  - Deng X
FAU - Huang, H
AU  - Huang H
FAU - Yu, L
AU  - Yu L
FAU - Zhao, S
AU  - Zhao S
FAU - Zheng, Q
AU  - Zheng Q
FAU - Adler, I
AU  - Adler I
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (DNA Probes)
SB  - IM
MH  - Cell Nucleus
MH  - *Chromosomes, Human, Pair 21
MH  - *DNA Probes
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Male
EDAT- 1999/02/09 00:00
MHDA- 1999/02/09 00:01
CRDT- 1999/02/09 00:00
PHST- 1999/02/09 00:00 [pubmed]
PHST- 1999/02/09 00:01 [medline]
PHST- 1999/02/09 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1999 Feb 10;16(1):36-40.