PMID- 9950822
OWN - NLM
STAT- MEDLINE
DCOM- 19990330
LR  - 20190212
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 276
IP  - 2
DP  - 1999 Feb
TI  - Role of endotoxin in intestinal reperfusion-induced expression of E-selectin.
PG  - G479-84
LID - 10.1152/ajpgi.1999.276.2.G479 [doi]
AB  - Products of enteric bacteria, including endotoxin [lipopolysaccharide (LPS)], 
      have been implicated in the acute inflammatory responses elicited by ischemia and 
      reperfusion (I/R) of the small intestine. The objective of this study was to 
      assess the contribution of LPS to the increased E-selectin expression observed in 
      the intestinal vasculature after I/R. The dual radiolabeled monoclonal antibody 
      technique was used in LPS-sensitive (C3HeB/FeJ) and LPS-insensitive (C3H/HeJ) 
      mice that were exposed to either exogenous LPS or to gut I/R (45 min ischemia, 5 
      h reperfusion). LPS elicited a dose-dependent (0.5-50 microgram LPS/animal) 
      increase in E-selectin expression (at 3 h) in LPS-sensitive mice, whereas 
      LPS-insensitive mice were largely unresponsive. E-selectin expression was 
      increased fivefold by I/R in the small bowel of both LPS-sensitive and 
      -insensitive mice. These results indicate that, although exogenous LPS is capable 
      of eliciting profound dose-dependent increases in E-selectin expression, 
      endogenous LPS does not contribute significantly to I/R-induced expression of 
      this endothelial cell adhesion molecule.
FAU - Bauer, P
AU  - Bauer P
AD  - Department of Molecular and Cellular Physiology, Louisiana State University 
      Medical Center, Shreveport, Louisiana 71130-3932, USA.
FAU - Russell, J M
AU  - Russell JM
FAU - Granger, D N
AU  - Granger DN
LA  - eng
GR  - P01-DK-43785/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (E-Selectin)
RN  - 0 (Endotoxins)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Drug Resistance/genetics
MH  - E-Selectin/*physiology
MH  - Endotoxins/blood/*physiology
MH  - Intestines/*blood supply
MH  - Ischemia/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H/genetics
MH  - Reperfusion Injury/*metabolism
EDAT- 1999/02/10 00:00
MHDA- 1999/02/10 00:01
CRDT- 1999/02/10 00:00
PHST- 1999/02/10 00:00 [pubmed]
PHST- 1999/02/10 00:01 [medline]
PHST- 1999/02/10 00:00 [entrez]
AID - 10.1152/ajpgi.1999.276.2.G479 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Feb;276(2):G479-84. doi: 10.1152/ajpgi.1999.276.2.G479.