PMID- 9951327
OWN - NLM
STAT- MEDLINE
DCOM- 19990309
LR  - 20150514
IS  - 0002-5151 (Print)
IS  - 0002-5151 (Linking)
VI  - 45
IP  - 6
DP  - 1998 Nov-Dec
TI  - [Diagnostic use of enzymatic RAST skin tests and determination of eosinophils in 
      nasal mucosa in allergic rhinitis].
PG  - 150-8
AB  - AIMS: Allergic rhinitis is the most frequent disease mediated by immunoglobulin E 
      (IgE). Nasal challenge is the gold standard for the diagnosis of allergic 
      rhinitis. Skin tests (ST) are the most used diagnostic method to detect the 
      presence of specific IgE bind to skin mast cells. The exposition of the nasal 
      mucous membrane to the allergen is followed by an increase of the local 
      eosinophils; the count of eosinophils in nasal mucous (ENM) is a diagnostic test 
      for allergic rhinitis. Enzymatic RAST or enzymatic allergo-sorbent test (ESA) 
      measures the level of serum allergen-specific IgE. OBJECTIVE: To measure the 
      sensitivity, specificity, and diagnostic precision of ST, EAST and ENM in 
      allergic rhinitis. METHOD: We studied 241 individuals, 162 of them had allergic 
      rhinitis, and 79 were healthy controls. They underwent nasal challenge and 
      intradermic ST for Dermatophagoies spp (acarus). Fraxinus americana (Ash-tree), 
      Amaranthus palmieri (quelite), Cynodon Dactylon (capriola) and Felis catus (cat), 
      EAST for Dermatophagoides pteronyssinus (acarus), and ENIVI. Results of ST, EAST 
      and ENIVI were compared with their corresponding nasal challenge, and the 
      prevalence of allergic rhinitis for each allergen was calculated. The best cut 
      point was assessed by means of receiver-operator curves (ROC), and sensitivity, 
      specificity, positive predictive value, negative predictive value, inter-observer 
      concordance coefficient, area under ROC (0), standard error of 0 (SEO), and 95% 
      confidence interval of 0 of each test were calculated using the best cut point. 
      RESULTS: ST and EAST had the best sensitivity and specificity. ENM had the lowest 
      sensitivity and specificity. CONCLUSION: For the diagnosis of Dermatophagoides 
      spp allergic rhinitis ST for Dermatophagoides spp and EAST for Dermatophagoides 
      pteronyssinus have the same diagnostic precision. According to the indexes for 
      diagnostic precision, and inter-observer concordance coefficient, ST and EAST are 
      useful to diagnose allergic rhinitis induced by the evaluated allergens. ENIVI is 
      a test that is not very useful for the diagnosis of allergic rhinitis.
FAU - Gomez Castillo, C A
AU  - Gomez Castillo CA
AD  - Departamento de inmunologia clinica y alergia, Hospital de Especialidades, Centro 
      Medico Nacional Siglo XXI, IMSS, Mexico, DF.
FAU - Martinez Cairo Cueto, S
AU  - Martinez Cairo Cueto S
LA  - spa
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
TT  - Utilidad diagnostica de las pruebas cutaneas RAST enzimatico y determinacion de 
      eosinofilos en moco nasal en la rinitis alergica.
PL  - Mexico
TA  - Rev Alerg Mex
JT  - Revista alergia Mexico (Tecamachalco, Puebla, Mexico : 1993)
JID - 9438824
SB  - IM
MH  - Eosinophils
MH  - Humans
MH  - Leukocyte Count
MH  - Nasal Mucosa/immunology
MH  - Nasal Provocation Tests
MH  - ROC Curve
MH  - Radioallergosorbent Test
MH  - Rhinitis, Allergic, Perennial/*diagnosis
MH  - Sensitivity and Specificity
MH  - Skin Tests/*methods
EDAT- 1999/02/10 00:00
MHDA- 1999/02/10 00:01
CRDT- 1999/02/10 00:00
PHST- 1999/02/10 00:00 [pubmed]
PHST- 1999/02/10 00:01 [medline]
PHST- 1999/02/10 00:00 [entrez]
PST - ppublish
SO  - Rev Alerg Mex. 1998 Nov-Dec;45(6):150-8.